The GH3 family in plants: Genome wide analysis in rice and evolutionary history based on EST analysis

The GH3 gene family in Arabidopsis, implicated in hormonal homeostasis through the conjugation of indolacetic and jasmonic acids to amino acids, is involved in a broad range of plant growth and development processes. In this work, the analysis of the GH3 family in the genome of Oryza sativa identifi...

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Bibliographic Details
Published in:Gene 2006-04, Vol.371 (2), p.279-290
Main Authors: Terol, Javier, Domingo, Concha, Talón, Manuel
Format: Article
Language:English
Subjects:
Arabidopsis thaliana
Brassica napus
Bryophyta
Chlorophyta
Coniferophyta
EST analysis
Evolution, Molecular
Expressed Sequence Tags
Genome, Plant
GH3 genes
Gossypium hirsutum
Magnoliophyta
Open Reading Frames
Oryza - genetics
Oryza sativa
Phylogenetic history
Plant Proteins - genetics
Reverse Transcriptase Polymerase Chain Reaction
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Summary:The GH3 gene family in Arabidopsis, implicated in hormonal homeostasis through the conjugation of indolacetic and jasmonic acids to amino acids, is involved in a broad range of plant growth and development processes. In this work, the analysis of the GH3 family in the genome of Oryza sativa identified 13 hypothetical ORFs. EST analysis and RT-PCR assays demonstrated that 12 of them were active genes. An extensive EST analysis of the GH3 family performed on 26 plant species was used to estimate the minimum number of GH3 genes en each one. The data indicated that the members of the GH3 family progressively increased in the different plant divisions from Chlorophyta (0), Bryophyta (3), and Coniferophyta (4), to Magnoliophyta (7–19). Phylogenetic analyses showed a high degree of conservation between Arabidopsis and rice GH3 proteins and, in general, in the plant kingdom. The data revealed a homology clustering consistent with the functional classification of the Arabidopsis proteins, since most of the 110 sequences analyzed grouped into 2 main clusters, corresponding to the Arabidopsis functional groups I (jasmonic acid adenylation) and II (indolacetic acid adenylation). And additional cluster including group III (non-adenylation ability) was exclusively composed of proteins from Arabidopsis thaliana, Brassica napus and Gossypium hirsutum.
ISSN:0378-1119
1879-0038
DOI:10.1016/j.gene.2005.12.014