Genomic characterisation of the toxin Amm VIII from the scorpion Androctonus mauretanicus mauretanicus

The genomic DNA sequence encoding the scorpion toxin Amm VIII was amplified from genomic DNA of the scorpion Androctonus mauretanicus mauretanicus from Morocco, subcloned and sequenced. An intron, with a high A+T content (73.5%), split a Gly codon at the end of the precursor signal peptide and the c...

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Bibliographic Details
Published in:Toxicon (Oxford) 2006-04, Vol.47 (5), p.531-536
Main Authors: Alami, Meriem, Céard, Brigitte, Legros, Christian, Bougis, Pierre E., Martin-Eauclaire, Marie-France
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Androctonus mauretanicus mauretanicus
Animal poisons toxicology. Antivenoms
Animals
Base Sequence
Biological and medical sciences
DNA
Intron
Introns
Leiurus quinquestriatus quinquestriatus
Medical sciences
Molecular Sequence Data
Scorpion
Scorpion Venoms - chemistry
Scorpion Venoms - genetics
Scorpions - chemistry
Scorpions - classification
Scorpions - genetics
Toxicology
Toxin
Voltage-activated sodium channel
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Summary:The genomic DNA sequence encoding the scorpion toxin Amm VIII was amplified from genomic DNA of the scorpion Androctonus mauretanicus mauretanicus from Morocco, subcloned and sequenced. An intron, with a high A+T content (73.5%), split a Gly codon at the end of the precursor signal peptide and the consensus GT/AG splice junction was identified in the Amm VIII gene. This intron of only 166 bp is the smallest intron described so far for a long-chain scorpion toxin gene. In addition, this study led to the identification of three new toxin-related genes. From the deduced amino acid sequences of the encoded precursor proteins, we found that the mature putative toxins were highly similar to the scorpion toxins Leiurus quinquestriatus quinquestriatus IV and Odonthobuthus doriae 1.
ISSN:0041-0101
1879-3150
DOI:10.1016/j.toxicon.2006.01.005