Evaluation of two TaqMan PCR assays for the detection of HIV-1 proviral DNA in blood samples

This study compared two published TaqMan PCR assays targeting different regions of the HIV-1 genome for detection of HIV-1 proviral DNA. The gag specific PCR demonstrated a lower sensitivity than the assay targeting the LTR region. The LTR assay is a highly reproducible and specific technique for HI...

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Bibliographic Details
Published in:Journal of microbiological methods 2006-05, Vol.65 (2), p.350-353
Main Authors: Moroney, Shannon M., Heller, Loree C., Widen, Ray H.
Format: Article
Language:English
Subjects:
Biological and medical sciences
DNA, Viral - blood
Fundamental and applied biological sciences. Psychology
gag
Genes, gag
HIV Infections - diagnosis
HIV Infections - virology
HIV Long Terminal Repeat
HIV-1 - genetics
HIV-1 - isolation & purification
HIV-1 proviral DNA
Human immunodeficiency virus 1
Humans
LTR
Microbiology
Polymerase Chain Reaction - methods
Proviruses - genetics
Proviruses - isolation & purification
Reproducibility of Results
Sensitivity and Specificity
Taq Polymerase
TaqMan PCR assays
Techniques used in virology
Virology
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Summary:This study compared two published TaqMan PCR assays targeting different regions of the HIV-1 genome for detection of HIV-1 proviral DNA. The gag specific PCR demonstrated a lower sensitivity than the assay targeting the LTR region. The LTR assay is a highly reproducible and specific technique for HIV-1 proviral DNA detection.
ISSN:0167-7012
1872-8359
DOI:10.1016/j.mimet.2005.08.003