Supercritical fluid chromatography tandem-column method development in pharmaceutical sciences for a mixture of four stereoisomers

A tandem‐column method using Chiralpak AD‐H and Chiralcel OD‐H columns was achieved for baseline separation of a mixture of chiral pharmaceutical compounds (i. e., four stereoisomers) via supercritical fluid chromatography (SFC) with a mobile phase consisting of 90% liquid carbon dioxide and 10% eth...

Full description

Saved in:
Bibliographic Details
Published in:Journal of separation science 2005-05, Vol.28 (7), p.619-626
Main Authors: Barnhart, Wesley W., Gahm, Kyung H., Thomas, Sam, Notari, Steve, Semin, David, Cheetham, Janet
Format: Article
Language:English
Subjects:
Analytical chemistry
Biological and medical sciences
Chemistry
Chromatographic methods and physical methods associated with chromatography
Chromatography, Liquid - instrumentation
Chromatography, Liquid - methods
Diastereomer
Exact sciences and technology
General pharmacology
Medical sciences
Other chromatographic methods
Pharmaceutical Preparations - chemistry
Pharmaceutical technology. Pharmaceutical industry
Pharmacology. Drug treatments
Stereoisomerism
Supercritical fluid chromatography
Tandem-column
Technology, Pharmaceutical - methods
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A tandem‐column method using Chiralpak AD‐H and Chiralcel OD‐H columns was achieved for baseline separation of a mixture of chiral pharmaceutical compounds (i. e., four stereoisomers) via supercritical fluid chromatography (SFC) with a mobile phase consisting of 90% liquid carbon dioxide and 10% ethanol:isopropanol (50 : 50 v/v). On the contrary, this mixture (mixture A) could not be baseline separated by SFC conditions explored with individual Chiralpak AD‐H and Chiralcel OD‐H columns. The effects of various mobile phases on elution order, capacity factor, selectivity, and resolution were determined with mixture A on the individual aforementioned columns to develop the tandem‐column method.
ISSN:1615-9306
1615-9314
DOI:10.1002/jssc.200500005