Protein expression dynamics during replicative senescence of endothelial cells studied by 2-D difference in-gel electrophoresis

• Published in: Electrophoresis 2006-04, Vol.27 (8), p.1669-1682
• Main Authors: Eman, Michael R., Regan-Klapisz, Elsa, Pinkse, Martijn W. H., Koop, Inge M., Haverkamp, Johan, Heck, Albert J. R., Verkleij, Arie J., Post, Jan A.
• Format: Article
• Language: English
• Subjects: Cell Proliferation - drug effects; Cells, Cultured; Cellular Senescence - physiology; Cytoskeleton - physiology; Difference in-gel electrophoresis; DNA Repair - physiology; Electrophoresis, Gel, Two-Dimensional - methods; Endothelial cell; Endothelial Cells - physiology; Humans; Nuclear integrity; Oxidative stress; Oxidative Stress - physiology; Protein Biosynthesis - physiology; Replicative senescence; Umbilical Veins - cytology
• ISSN: 0173-0835; 1522-2683
• DOI: 10.1002/elps.200500746

Endothelial senescence contributes to endothelium dysfunctionality and is thereby linked to vascular aging. A dynamic proteomic study on human umbilical vein endothelial cells, isolated from three umbilical cords, was performed. The cells were cultured towards replicative senescence and whole cell lysates were subjected to 2‐D difference gel electrophoresis (DIGE). Despite the biological variability of the three independent isolations, a set of proteins was found that showed senescence‐dependent expression patterns in all isolations. We focused on those proteins that showed significant changes, with a paired analysis of variance (RM‐ANOVA) p‐value of ≤0.05. Thirty‐five proteins were identified with LC‐Fourier transform MS, and functional annotation revealed that endothelial replicative senescence is accompanied by increased cellular stress, protein biosynthesis and reduction in DNA repair and maintenance. Nuclear integrity becomes affected and cytoskeletal structure is also changed. Such important changes in the cell infrastructure might accelerate endothelium dysfunctionality. This study provides biological information that will initiate studies to further unravel endothelial senescence and gain more knowledge about the consequences of this process in the in vivo situation.