Beet Sugar Syrup and Molasses as Low-Cost Feedstock for the Enzymatic Production of Fructo-oligosaccharides

Sugar syrup and molasses from beet processing containing 620 and 570 mg/mL sucrose, respectively, were assayed as low-cost and available substrates for the enzymatic synthesis of fructo-oligosaccharides (FOSs). A commercial pectinase (Pectinex Ultra SP-L, from Aspergillus aculeatus) characterized by...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2006-04, Vol.54 (8), p.2964-2968
Main Authors: Ghazi, Iraj, Fernandez-Arrojo, Lucia, Gomez De Segura, Aranzazu, Alcalde, Miguel, Plou, Francisco J, Ballesteros, Antonio
Format: Article
Language:English
Subjects:
Animal Feed
Aspergillus - enzymology
Aspergillus aculeatus
beet sugar
Beta vulgaris - chemistry
Biological and medical sciences
Carbohydrates
endopectinylase
enzymatic synthesis
enzymatic treatment
Enzymes, Immobilized
feed additives
Feed and pet food industries
feedstocks
Food industries
fructooligosaccharides
Fundamental and applied biological sciences. Psychology
Hexosyltransferases - metabolism
Hydrogen-Ion Concentration
immobilized enzymes
Kinetics
Molasses
Oligosaccharides - biosynthesis
pectinesterase
polygalacturonase
Polygalacturonase - metabolism
Probiotics
Sugar industries
synthesis
syrups
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Summary:Sugar syrup and molasses from beet processing containing 620 and 570 mg/mL sucrose, respectively, were assayed as low-cost and available substrates for the enzymatic synthesis of fructo-oligosaccharides (FOSs). A commercial pectinase (Pectinex Ultra SP-L, from Aspergillus aculeatus) characterized by the presence of a transfructosylating activity was used as a biocatalyst. The FOS production increased when lowering the initial pH value of syrup (7.5) and molasses (8.9) to 5.5. Sugar syrup and molasses were diluted in order to reduce substrate viscosity; interestingly, the percentage of FOS with regards to total sugars remained almost constant, which indicated a high transferase-to-hydrolase ratio for this enzyme. Kinetics of FOS production was analyzed. Using approximately 10 U transfructosylating activity per g sucrose, the FOS concentration reached a maximum of 388 mg/mL after 30 h using syrup and 235 mg/mL in 65 h with molasses. These values corresponded to approximately 56 and 49% (w/w), respectively, of the total amount of carbohydrates in the mixture. The enzyme was also covalently immobilized on an epoxy-activated polymethacrylate-based polymer (Sepabeads EC-EP5). We found that immobilized Pectinex Ultra SP-L can be efficiently applied to the synthesis of FOS using syrup and molasses as substrates. Keywords: β-Fructofuranosidase; fructosyltransferase; transglycosidase; sucrose; prebiotics; polymethacrylate; Pectinex Ultra SP-L; animal feed
ISSN:0021-8561
1520-5118
DOI:10.1021/jf053023b