Simultaneous observation of collagen and elastin in normal and pathological tissues: analysis of Sirius-red-stained sections by fluorescence microscopy

In order to observe collagen and elastic fibers simultaneously, sections of human aorta, skin, lung, liver, and bladder were stained by Sirius red and analyzed by fluorescence microscopy. In all cases, the fibers of collagen presented the characteristic fluorescent red-orange color that results from...

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Bibliographic Details
Published in:Cell and tissue research 2005-06, Vol.320 (3), p.551-552
Main Authors: Borges, L F, Taboga, S R, Gutierrez, P S
Format: Article
Language:English
Subjects:
Aorta - anatomy & histology
Aorta - metabolism
Azo Compounds - chemistry
Collagen - chemistry
Collagen - metabolism
Coloring Agents - chemistry
Elastin - metabolism
Fibrosis
Humans
Liver - metabolism
Lung - metabolism
Microscopy, Fluorescence
Myocardium - metabolism
Myocardium - pathology
Organ Specificity
Skin - anatomy & histology
Skin - metabolism
Urinary Bladder - metabolism
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Summary:In order to observe collagen and elastic fibers simultaneously, sections of human aorta, skin, lung, liver, and bladder were stained by Sirius red and analyzed by fluorescence microscopy. In all cases, the fibers of collagen presented the characteristic fluorescent red-orange color that results from the interaction of this extracellular protein with the dye, whereas elastic fibers showed strong green fluorescence (intrinsic fluorescence). This method efficiently detects collagen and elastic fibers when these two structures are present and could have valuable applications in processes that involves both fibers.
ISSN:0302-766X
1432-0878
DOI:10.1007/s00441-005-1108-6