Arising Podosomal Structures are Associated with Neoplastic Cell Morphological Phenotype Induced by the Microenvironment

Increased numbers of rosettes of podosomes were observed in overgrown rat Rous sarcoma RsK4 cells. A possible role of these structures in nutrient uptake in tumour cell survival was investigated by exposure to acute starvation. A single cell suspension of RsK4 cells in Hanks balanced salt solution w...

Full description

Saved in:
Bibliographic Details
Published in:Anticancer research 2006-03, Vol.26 (2A), p.967-972
Main Authors: VESELY, Pavel, BLASE, Christopher, MATOUSKOVA, Eva, BEREITER-HAHN, Jürgaen
Format: Article
Language:English
Subjects:
Actins - metabolism
Animal tumors. Experimental tumors
Animals
Biological and medical sciences
Cell Movement - physiology
Cell Surface Extensions - metabolism
Cell Surface Extensions - physiology
Culture Media
Medical sciences
Microscopy, Confocal
Rats
Rats, Inbred Lew
Sarcoma, Avian - metabolism
Sarcoma, Avian - pathology
Spontaneous animal tumors
Tumors
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Increased numbers of rosettes of podosomes were observed in overgrown rat Rous sarcoma RsK4 cells. A possible role of these structures in nutrient uptake in tumour cell survival was investigated by exposure to acute starvation. A single cell suspension of RsK4 cells in Hanks balanced salt solution was allowed to interact with either clean uncoated or serum-coated for bait coverglasses. Confocal microscopy revealed contrasting 3D cell morphologies that were associated with conspicuous patterns of podosomal structures, which on the coated coverglasses resembled the sealing zones of osteoclasts, while on the uncoated coverglasses they resembled the marginal podosomes of migrating monocyte-derived cells. Thus, the arising podosomal structures, the involvement of which in an uptake of nutrients appeared feasible morphologically, were associated with the emerging 3D cell shapes guided by the microenvironment. Such phenotypic plasticity of neoplastic RsK4 cells in response to micro-environmental challenge suggested that uniqueness in cellular attributes within the neoplastic cell population could be crucial for the malignant potential.
ISSN:0250-7005
1791-7530