Peroxisomal localization of a myosin XI isoform in Arabidopsis thaliana

• Published in: Plant and cell physiology 2005-05, Vol.46 (5), p.782-789
• Main Authors: Hashimoto, K.(University of Hyogo, Kamigoori (Japan)), Igarashi, H, Mano, S, Nishimura, M, Shimmen, T, Yokota, E
• Format: Article
• Language: English
• Subjects: ACTIN; Actin Cytoskeleton - metabolism; ACTINA; ACTINE; Amino Acid Sequence; Arabidopsis; Arabidopsis - genetics; Arabidopsis - metabolism; Arabidopsis Proteins - chemistry; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; ARABIDOPSIS THALIANA; bovine serum albumin; BSA; CBB; CELL STRUCTURE; Coomassie brilliant blue; ESTRUCTURA CELULAR; EXPRESION GENICA; EXPRESSION DES GENES; FENOTIPOS; GENE EXPRESSION; GFP; green fluorescent protein; latrunculin B; MIOSINA; Molecular Sequence Data; Mutation - genetics; MYA2; MYOSIN; Myosin Heavy Chains - chemistry; Myosin Heavy Chains - genetics; Myosin Heavy Chains - metabolism; Myosin XI; MYOSINE; Myosins - chemistry; Myosins - genetics; Myosins - metabolism; OXIDOREDUCTASES; OXIDORREDUCTASAS; OXYDOREDUCTASE; PBS; Peroxisome; Peroxisomes - genetics; Peroxisomes - metabolism; PHENOTYPE; PHENOTYPES; phosphate-buffered saline; Plant Epidermis - cytology; Plant Epidermis - metabolism; Plant Leaves - cytology; Plant Leaves - metabolism; Plant Roots - cytology; Plant Roots - metabolism; PLANTAS TRANSGENICAS; PLANTE TRANSGENIQUE; Protein Isoforms - chemistry; Protein Isoforms - genetics; Protein Isoforms - metabolism; Protein Structure, Tertiary; Protein Transport - physiology; reverse transcription–polymerase chain reaction; RT–PCR; STRUCTURE CELLULAIRE; TRANSGENIC PLANTS; ULTAESTRUCTURA; ULTRASTRUCTURE
• ISSN: 0032-0781; 1471-9053
• DOI: 10.1093/pcp/pci085

The genome of Arabidopsis thaliana contains 13 myosin XI isoforms. Here we prepared a specific ahtibody against a peptide that mimics a unique C-terminal region from the myosin XI isoform, MYA2. The resulting antibody was used to demonstrate that MYA2 in Arabidopsis protein extracts co-sedimented with actin filaments and dissociated from the filaments with ATP treatment. Immunolocalization studies showed that MYA2 co-localized predominantly with actin filaments in clustered punctuate dots in leaf epidermal cells, root hair cells and suspension-cultured cells. In a transgenic plant in which peroxisomes are labeled with green fluorescent protein, some MYA2 signals were localized on peroxisomes in an actin-dependent manner. We propose that the peroxisome is one of the cargos translocated by MYA2 on actin filaments.