Genetic immunization with codon-optimized equine infectious anemia virus (EIAV) surface unit (SU) envelope protein gene sequences stimulates immune responses in ponies

In the context of DNA vaccines the native equine infectious anemia virus (EIAV)-envelope gene has proven to be an extremely weak immunogen in horses probably because the RNA transcripts are poorly expressed owing to an unusual codon-usage bias, the possession of multiple RNA splice sites and potenti...

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Bibliographic Details
Published in:Veterinary microbiology 2005-06, Vol.108 (1), p.23-37
Main Authors: Cook, R. Frank, Cook, Sheila J., Bolin, Pamela S., Howe, Laryssa J., Zhou, Weisong, Montelaro, Ronald C., Issel, Charles J.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
amino acid sequences
Animals
Antibodies, Viral - immunology
Base Sequence
Biological and medical sciences
Cell Proliferation
coat proteins
Codon-optimization
codons
DNA vaccine
DNA vaccines
EIAV
Equine Infectious Anemia - prevention & control
Equine infectious anemia virus
Fundamental and applied biological sciences. Psychology
genetic immunization
horse diseases
Horses
immune response
immunization
Immunoglobulin G - blood
Infectious Anemia Virus, Equine - immunology
messenger RNA
Microbiology
Miscellaneous
Molecular Sequence Data
nucleotide sequences
plasmids
Recombinant Proteins - immunology
RNA splicing
T-Lymphocytes
Time Factors
vaccines
Vaccines, DNA - immunology
viral diseases of animals and humans
Viral Envelope Proteins - chemistry
Viral Envelope Proteins - immunology
Viral Vaccines - immunology
Virology
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Summary:In the context of DNA vaccines the native equine infectious anemia virus (EIAV)-envelope gene has proven to be an extremely weak immunogen in horses probably because the RNA transcripts are poorly expressed owing to an unusual codon-usage bias, the possession of multiple RNA splice sites and potential adenosine-rich RNA instability elements. To overcome these problems a synthetic version of sequences encoding the EIAV surface unit (SU) envelope glycoprotein was produced (SYNSU) in which the codon-usage bias was modified to conform to that of highly expressed horse and human genes. In transfected COS-1 cell cultures, the steady state expression levels of SYNSU were at least 30-fold greater than equivalent native SU sequences. More importantly, EIAV-specific humoral and lymphocyte proliferation responses were induced in ponies immunized with a mammalian expression vector encoding SYNSU. However, these immunological responses were unable to confer protection against infection with a virulent EIAV strain.
ISSN:0378-1135
1873-2542
DOI:10.1016/j.vetmic.2005.04.004