Evaluation of IDA-PEVA hollow fiber membrane metal ion affinity chromatography for purification of a histidine-tagged human proinsulin

Inabilities to process particulate material and to allow the use of high flow rates are limitations of conventional chromatography. Membranes have been suggested as matrix for affinity separation due to advantages such as allowing high flow rates and low-pressure drops. This work evaluated the feasi...

Full description

Saved in:
Bibliographic Details
Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2006-04, Vol.834 (1), p.68-76
Main Authors: de Aquino, Luciana Cristina Lins, de Sousa, Heloisa Ribeiro Tunes, Miranda, Everson Alves, Vilela, Luciano, Bueno, Sônia Maria Alves
Format: Article
Language:English
Subjects:
Adsorption
Affinity membrane
Analysis
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Chromatography, Affinity - methods
Electrophoresis, Polyacrylamide Gel
Fundamental and applied biological sciences. Psychology
General pharmacology
Histidine - chemistry
Humans
IMAC
Medical sciences
Membranes, Artificial
Metals - chemistry
Pharmacology. Drug treatments
Proinsulin - chemistry
Proinsulin - isolation & purification
Proinsulin-(His) 6
Purification
Thermodynamics
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Inabilities to process particulate material and to allow the use of high flow rates are limitations of conventional chromatography. Membranes have been suggested as matrix for affinity separation due to advantages such as allowing high flow rates and low-pressure drops. This work evaluated the feasibility of using an iminodiacetic acid linked poly(ethylenevinyl alcohol) membrane in the immobilized metal ion affinity chromatography (IMAC) purification of a human proinsulin(His) 6 of an industrial insulin production process. The screening of metal ions showed Ni 2+ as metal with higher selectivity and capacity among the Cu 2+, Ni 2+, Zn 2+ and Co 2+. The membrane showed to be equivalent to conventional chelating beads in terms of selectivity and had a lower capacity (3.68 mg/g versus 12.26 mg/g). The dynamic adsorption capacity for human proinsulin(His) 6 was unaffected by the mode of operation (dead-end and cross-flow filtration).
ISSN:1570-0232
1873-376X
DOI:10.1016/j.jchromb.2006.02.015