A movement protein and three capsid proteins are all necessary for the cell-to-cell movement of apple latent spherical cheravirus

Immunoblot analysis of apple latent spherical cheravirus (ALSV)-infected leaves using a polyclonal antibody against the 21 C-terminal amino acids of a 53 K/42 K movement protein (MP) showed that a protein with an Mr of 42 kDa (42KP) is the dominant form found in vivo, which could indicate that the s...

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Bibliographic Details
Published in:Archives of virology 2006-05, Vol.151 (5), p.837-848
Main Authors: YOSHIKAWA, N, OKADA, K, ASAMUMA, K, WATANABE, K, IGARASI, A, LI, C, ISOGAI, M
Format: Article
Language:English
Subjects:
Amino acids
Biological and medical sciences
Blotting, Western
Capsid Proteins - physiology
Cloning
DNA Mutational Analysis
Enzymes
Fundamental and applied biological sciences. Psychology
Genes, Reporter
Green Fluorescent Proteins - genetics
Green Fluorescent Proteins - metabolism
Leaves
Malus
Malus - virology
Microbiology
Microscopy, Electron, Transmission
Microscopy, Fluorescence
Miscellaneous
Molecular Weight
Organelles - ultrastructure
Organelles - virology
Plant Leaves - ultrastructure
Plant Leaves - virology
Plant Viral Movement Proteins
Plant Viruses - physiology
Polyclonal antibodies
Polypeptides
Proteins
RNA polymerase
RNA Viruses - physiology
Sequence Deletion
Viral Proteins - analysis
Viral Proteins - physiology
Virology
Viruses
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Summary:Immunoblot analysis of apple latent spherical cheravirus (ALSV)-infected leaves using a polyclonal antibody against the 21 C-terminal amino acids of a 53 K/42 K movement protein (MP) showed that a protein with an Mr of 42 kDa (42KP) is the dominant form found in vivo, which could indicate that the second AUG is used as an initiation codon of a ORF in RNA2. Co-expression of GFP with 42KP in tobacco epidermal cells showed that 42KP is able to facilitate cell-to-cell trafficking of GFP that is expressed in the same cells. The analysis of deletion mutants on each of MP, Vp24, Vp20, or Vp25 using an ALSV vector that stably expresses GFP indicated that an MP and three capsid proteins are all indispensable for the cell-to-cell movement of the virus. In ultrathin sections of infected leaves, a file of virus-like particles passing through the plasmodesmata connecting neighboring cells and tubular structures containing virus-like particles extending into the cytoplasm were observed. These results show that ALSV moves from cell to cell as virus particles.
ISSN:0304-8608
1432-8798
DOI:10.1007/s00705-005-0689-4