mRNA up-regulation of MHC II and pivotal pro-inflammatory genes in normal brain aging

In normal brain aging, CNS resident macrophages exhibit increased expression of major histocompatibility complex (MHC) II expression. However, the transcriptional basis for this observation has not been clarified nor have age-related alterations in pivotal pro-inflammatory genes been characterized....

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Bibliographic Details
Published in:Neurobiology of aging 2006-05, Vol.27 (5), p.717-722
Main Authors: Frank, Matthew G., Barrientos, Ruth M., Biedenkapp, Joseph C., Rudy, Jerry W., Watkins, Linda R., Maier, Steven F.
Format: Article
Language:English
Subjects:
Aging
Aging - genetics
Animals
Antigens, CD - genetics
Brain - growth & development
DNA Primers
DNA, Complementary - biosynthesis
DNA, Complementary - genetics
Gene Expression Regulation, Developmental - physiology
Genes, MHC Class II - genetics
Hippocampus
Immune
Inflammation - genetics
Macrophage
Male
MHC II
Microglia
Neurons - physiology
Pro-inflammatory
Rats
Rats, Inbred F344
Receptors, Immunologic - genetics
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - biosynthesis
RNA, Messenger - isolation & purification
Up-Regulation - physiology
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Summary:In normal brain aging, CNS resident macrophages exhibit increased expression of major histocompatibility complex (MHC) II expression. However, the transcriptional basis for this observation has not been clarified nor have age-related alterations in pivotal pro-inflammatory genes been characterized. Age-related mRNA alterations in MHC II, MHC II accessory molecules and several pro-inflammatory mediators were measured in older (24 months) and younger (3 months) male F344xBN F1 rats. Real time RT-PCR was utilized to measure steady state mRNA levels in hippocampus. Older as compared to younger animals exhibited increased mRNA levels of MHC II, CD86, CIITA and IFN-γ. Furthermore, IL-10 and CD200 mRNA, molecules that down-regulate macrophage activation, was decreased in older animals. The present results indicate that normal brain aging is characterized by a shift towards a pro-inflammatory microenvironment in the CNS.
ISSN:0197-4580
1558-1497
DOI:10.1016/j.neurobiolaging.2005.03.013