Rapid determination of trace amounts of minoxidil in hamster skin follicles with various formulations using narrow-bore LC/EC

A sensitive liquid chromatographic method with electrochemistry (LC/EC) was developed for the determination of trace of minoxidil in hamster skin follicles after topical administration of the ear using various formulations. The minoxidil in the sebaceous glands of the hamster ear was isolated from t...

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Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 2005-07, Vol.38 (3), p.532-536
Main Authors: Huang, Tiehua, Garceau, Michelle E., Ramstad, Tore, Stehle, Randall G.
Format: Article
Language:English
Subjects:
Analysis
Analytical, structural and metabolic biochemistry
Animals
Biological and medical sciences
Chromatography, High Pressure Liquid - methods
Cricetinae
Dermis - metabolism
Dosage Forms
Ear
Electrochemical detection
Electrochemistry - methods
Follicles
Formulation
Fundamental and applied biological sciences. Psychology
General pharmacology
Hydrogen-Ion Concentration
Medical sciences
Microbore liquid chromatography
Minoxidil
Minoxidil - analysis
Minoxidil - isolation & purification
Minoxidil - pharmacokinetics
Pharmacology. Drug treatments
Polyethylene Glycols - chemistry
Reproducibility of Results
Sebaceous Glands - metabolism
Solubility
Solvents - chemistry
Trichloroacetic Acid - chemistry
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Summary:A sensitive liquid chromatographic method with electrochemistry (LC/EC) was developed for the determination of trace of minoxidil in hamster skin follicles after topical administration of the ear using various formulations. The minoxidil in the sebaceous glands of the hamster ear was isolated from the skin and the follicles in different skin layers were treated with aqueous trichloroacetic acid followed by acetonitrile. The supernatant was directly injected into the LC/EC system and minoxidil was detected by oxidation at +800 mV versus Ag/AgCl using a glassy carbon electrode. The analytical recoveries were between 94.4 and103.1% and the linearity was excellent up to 250 μg/ml with a regression coefficient ( r 2) of 0.9988. The LC/EC and the widely used radiolabeled scintillation methods agree well and both show high sensitivities. The LC/EC method is rapid and cost-effective with a detection limit of only 1 ng/ml.
ISSN:0731-7085
1873-264X
DOI:10.1016/j.jpba.2005.01.032