Evidence that IncG (IncP-6) and IncU plasmids form a single incompatibility group

The Escherichia coli IncG and IncU plasmid incompatibility groups were assigned in 1980 and 1981, respectively. Complete plasmid sequences have recently been published for both these groups, and revealed that their replication proteins are related. We show that when cloned at high-copy-number, putat...

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Bibliographic Details
Published in:Plasmid 2006-05, Vol.55 (3), p.210-215
Main Authors: Haines, Anthony S., Cheung, Martin, Thomas, Christopher M.
Format: Article
Language:English
Subjects:
Broad host-range
DNA Replication
DNA, Bacterial - genetics
Escherichia coli
Escherichia coli - genetics
Incompatibility group
Iteron
Plasmids
Pseudomonas
Replicon
Segregation
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Summary:The Escherichia coli IncG and IncU plasmid incompatibility groups were assigned in 1980 and 1981, respectively. Complete plasmid sequences have recently been published for both these groups, and revealed that their replication proteins are related. We show that when cloned at high-copy-number, putative iterons from the previously identified IncG replicon cause strong incompatibility with IncU plasmids. Incompatibility, albeit weaker, was also demonstrated between the two replicons at their normal low-copy-number. This suggests that a single incompatibility group exists. The only known IncG plasmid, Rms149, can replicate in Pseudomonas species where it is designated IncP-6. We recommend that the combined group be known as IncU (IncP-6 in Pseudomonas spp.).
ISSN:0147-619X
1095-9890
DOI:10.1016/j.plasmid.2005.11.003