Capillary electrophoresis assay of netilmicin sulphate

An effective method based on capillary electrophoresis (CE) for the determination of netilmicin sulphate in commercial ophthalmic formulations was developed and validated. The use of a polymer-coated capillary and a non-absorbing running buffer permitted the elution of netilmicin in cationic mode at...

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Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 2005-06, Vol.38 (2), p.344-348
Main Authors: Calcara, Maria, Enea, Vincenzo, Pricoco, Angelo, Miano, Fausto
Format: Article
Language:English
Subjects:
Aminoglycosides
Analysis
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Capillary electrophoresis
Electrophoresis, Capillary - instrumentation
Electrophoresis, Capillary - methods
Fundamental and applied biological sciences. Psychology
General pharmacology
Hydrogen-Ion Concentration
Medical sciences
Netilmicin
Netilmicin - analysis
Netilmicin - chemistry
Pharmacology. Drug treatments
Polyethylene Glycols - chemistry
Polyvinyl Alcohol - chemistry
Reproducibility of Results
Technology, Pharmaceutical - methods
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Summary:An effective method based on capillary electrophoresis (CE) for the determination of netilmicin sulphate in commercial ophthalmic formulations was developed and validated. The use of a polymer-coated capillary and a non-absorbing running buffer permitted the elution of netilmicin in cationic mode at pH 3.0 and with direct UV detection at λ = 195 nm. Since pre-treatment of the samples is not required, this procedure may be straightforwardly applied to the other aminoglycosides provided that their extinction coefficient is not too low.
ISSN:0731-7085
1873-264X
DOI:10.1016/j.jpba.2004.12.019