Transformation of Actinidia eriantha : A potential species for functional genomics studies in Actinidia

Protocols were developed for regeneration and Agrobacterium-mediated transformation of Actinidia eriantha Benth. A. eriantha has a number of features that make it a useful tool for functional genomics in Actinidia: the vines are relatively small and non-vigorous in nature, flowers form all over the...

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Bibliographic Details
Published in:Plant cell reports 2006-05, Vol.25 (5), p.425-431
Main Authors: TIANCHI WANG, YIDONG RAN, ATKINSON, Ross G, GLEAVE, Andrew P, COHEN, Dan
Format: Article
Language:English
Subjects:
Actinidia - genetics
Actinidia - metabolism
Biological and medical sciences
Biotechnology
Flowers
Flowers - genetics
Flowers - physiology
Fruit - genetics
Fruit - physiology
Fundamental and applied biological sciences. Psychology
Genetic engineering
Genetic technics
Genetic Vectors
Genomics
Glucuronidase - genetics
Glucuronidase - metabolism
Greenhouses
Methods. Procedures. Technologies
Phenotypes
Plant tissues
Plants, Genetically Modified - genetics
Plants, Genetically Modified - metabolism
Rhizobium - genetics
Seedlings
Transformation, Genetic
Transgenic animals and transgenic plants
Transgenic plants
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Summary:Protocols were developed for regeneration and Agrobacterium-mediated transformation of Actinidia eriantha Benth. A. eriantha has a number of features that make it a useful tool for functional genomics in Actinidia: the vines are relatively small and non-vigorous in nature, flowers form all over the vine including on lower axillary branches and the species flowers prolifically in greenhouse conditions. Flowering and fruiting of transgenic A. eriantha plants was obtained within 2 years of transformation in a containment greenhouse. GUS (beta-glucuronidase) activity indicating stable expression of the uidA gene was observed in leaf, stem, root, petal and fruit tissues. Molecular evidence for incorporation of transgenes into the A. eriantha genome was obtained by PCR and DNA gel blot analysis. Inheritance of transgenic phenotypes was demonstrated in seedling progeny. Functional genomic studies in kiwifruit have been initiated using transgenic A. eriantha plants.
ISSN:0721-7714
1432-203X
DOI:10.1007/s00299-005-0080-7