Biogenic amine and neuropeptide inputs to identified pelvic floor motoneurons that also express SRC-1

In the rat, the neurochemical phenotypes of neurons that are presynaptic to motoneurons innervating the levator ani are poorly defined. In this study, motoneurons within the spinal nucleus of the bulbospongiosus (SNB) were revealed, using retrograde labelling, following injection of cholera toxin B...

Full description

Saved in:
Bibliographic Details
Published in:Neuroscience letters 2005-07, Vol.382 (3), p.248-253
Main Authors: Ranson, Richard N., Santer, Robert M., Watson, Alan H.D.
Format: Article
Language:English
Subjects:
Animals
Biogenic Amines - metabolism
Biological and medical sciences
Dopamine beta hydroxylase
Dopamine beta-Hydroxylase - metabolism
Fundamental and applied biological sciences. Psychology
Histone Acetyltransferases
Immunohistochemistry
Lumbosacral Region
Male
Motor Neurons - cytology
Neuropeptides - metabolism
Nuclear Receptor Coactivator 1
Pelvic Floor - innervation
Rats
Rats, Wistar
Serotonin
Serotonin - metabolism
Spinal Cord - cytology
Spinal nucleus of the bulbospongiosus
Substance P
Substance P - metabolism
Transcription Factors - biosynthesis
Transcription Factors - metabolism
Vertebrates: nervous system and sense organs
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In the rat, the neurochemical phenotypes of neurons that are presynaptic to motoneurons innervating the levator ani are poorly defined. In this study, motoneurons within the spinal nucleus of the bulbospongiosus (SNB) were revealed, using retrograde labelling, following injection of cholera toxin B subunit into the levator ani muscle. Different classes of neuron making substantial inputs onto these labelled neurons were revealed by using immunocytochemistry for dopamine beta hydroxylase, serotonin and substance P. Appositions (sites of presumptive synapses) between immunoreactive terminals and both the somata and dendrites of labelled SNB motoneurons were commonly seen suggesting that substance P, noradrenaline and serotonin are likely to exert a significant influence on the activity of perineal motoneurons and thus on sexual reflexes. Additionally, steroid receptor coactivator-1 was found to be present in the nuclei of 96% of SNB neurons retrogradely labelled from the levator ani. This suggests that practically all of the neurons that innervate the levator ani are likely to be modulated by circulating steroid hormones.
ISSN:0304-3940
1872-7972
DOI:10.1016/j.neulet.2005.03.040