Expression of engrailed in the developing brain and appendages of the onychophoran euperipatoides kanangrensis (Reid)

We have cloned an engrailed‐class gene in the onychophoran Euperipatoides kanangrensis and investigated its expression using in situ hybridisation. The expression pattern was found to differ drastically from that previously described for another onychophoran species. In the present investigation, en...

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Bibliographic Details
Published in:Journal of experimental zoology. Part B, Molecular and developmental evolution Molecular and developmental evolution, 2005-05, Vol.304B (3), p.220-228
Main Authors: Eriksson, B. Joakim, Larson, Earl T., Thörnqvist, Per-Ove, Tait, Noel N., Budd, Graham E.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Biological Evolution
Brain - embryology
Brain - metabolism
DNA Primers
Extremities - embryology
Female
Gene Expression Regulation, Developmental
Homeodomain Proteins - genetics
Homeodomain Proteins - metabolism
In Situ Hybridization
Invertebrates - embryology
Molecular Sequence Data
Neurons - metabolism
New South Wales
Sequence Alignment
Sequence Analysis, DNA
Species Specificity
Transcription Factors - genetics
Transcription Factors - metabolism
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Summary:We have cloned an engrailed‐class gene in the onychophoran Euperipatoides kanangrensis and investigated its expression using in situ hybridisation. The expression pattern was found to differ drastically from that previously described for another onychophoran species. In the present investigation, engrailed transcripts were detected in a subset of developing neurons in the brain anlage, and in the mesoderm as well as ectoderm of the developing limb buds. The engrailed positive cells of the brain are of differing developmental maturity, ranging from subepidermal neuronal precursors to neurons located basally in the embryo with developing axons. The lack of the traditional expression in the posterior compartment of segments reported earlier in onychophorans is discussed, and we suggest that onychophorans may have acquired two copies of engrailed with different functions. J. Exp. Zool. 304B, 2005. © 2005 Wiley‐Liss, Inc.
ISSN:1552-5007
1552-5015
DOI:10.1002/jez.b.21043