Leukemic cell death induced by antithymocyte globulin

We studied the cytotoxic effects of antithymocyte globulin (ATG) in leukemic cells obtained from five patients with acute T lymphoblastic leukemia or precursor T lymphoblastic leukemia. ATG by itself killed leukemic cells in a dose-dependent manner. Simultaneous incubation with human AB or baby rabb...

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Bibliographic Details
Published in:Leukemia research 2005-07, Vol.29 (7), p.821-827
Main Authors: Yoshimi, Ayami, Ito, Masahumi, Kojima, Seiji
Format: Article
Language:English
Subjects:
Animals
Antilymphocyte Serum - pharmacology
Antithymocyte globulin
apoptosis
Caspase
Cell Death - drug effects
Cell Line, Tumor
Complement-mediated cytolysis
Fas antigen
Horses
Humans
Immunoglobulin G - pharmacology
Immunosuppressive Agents - pharmacology
Leukemia
Leukemia-Lymphoma, Adult T-Cell - immunology
Leukemia-Lymphoma, Adult T-Cell - pathology
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Summary:We studied the cytotoxic effects of antithymocyte globulin (ATG) in leukemic cells obtained from five patients with acute T lymphoblastic leukemia or precursor T lymphoblastic leukemia. ATG by itself killed leukemic cells in a dose-dependent manner. Simultaneous incubation with human AB or baby rabbit serum resulted in increased cytolysis of leukemic cells. These results suggested the presence of both direct and complement-mediated cytolysis. We also examined apoptotic cell death using Annexin-V. Cell incubation with ATG increased Annexin-V binding significantly compared with horse IgG (50.3 ± 7.6% versus 95.7 ± 1.8%, p =
ISSN:0145-2126
1873-5835
DOI:10.1016/j.leukres.2002.11.001