Borrelia burgdorferi sensu stricto invasiveness is correlated with OspC–plasminogen affinity

Borrelia burgdorferi sensu lato, the causative agent of Lyme borreliosis, is transmitted through tick bite. Lyme borreliosis evolves in two stages: a primary red skin lesion called erythema migrans; later on, invasive bacteria disseminate to distant sites inducing secondary manifestations (neuropath...

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Bibliographic Details
Published in:Microbes and infection 2006-03, Vol.8 (3), p.645-652
Main Authors: Lagal, Vanessa, Portnoï, Denis, Faure, Grazyna, Postic, Danièle, Baranton, Guy
Format: Article
Language:English
Subjects:
Animals
Antigens, Bacterial - metabolism
Bacterial Outer Membrane Proteins - metabolism
Bacteriology
Biological and medical sciences
Borrelia burgdorferi
Borrelia burgdorferi - physiology
Borrelia burgdorferi sensu stricto
Fundamental and applied biological sciences. Psychology
Humans
Lyme Disease - microbiology
Mice
Mice, SCID
Microbiology
Miscellaneous
OspC
Plasminogen
Plasminogen - metabolism
Protein Binding
Specific Pathogen-Free Organisms
Surface plasmon resonance
Time Factors
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Summary:Borrelia burgdorferi sensu lato, the causative agent of Lyme borreliosis, is transmitted through tick bite. Lyme borreliosis evolves in two stages: a primary red skin lesion called erythema migrans; later on, invasive bacteria disseminate to distant sites inducing secondary manifestations (neuropathies, arthritis, carditis, late skin disorders). It has been previously suggested that the ospC gene could be associated with invasiveness in humans depending on its sequence. Here, we confirm the pattern of invasiveness, according to B. burgdorferi sensu stricto ( B. b. ss) ospC group, using the mouse as an experimental host of B. b. ss. As it has been shown that the host plasminogen activation system is used by B. burgdorferi to disseminate throughout the host, we studied the interaction of plasminogen with OspC proteins from invasive and non-invasive groups of B. b. ss. Using two methods, ELISA and surface plasmon resonance, we demonstrate that indeed OspC is a plasminogen-binding protein. Moreover, significant differences in binding affinity for plasminogen are correlated with different invasiveness patterns in mice. These results suggest that the correlation between ospC polymorphism and Borrelia invasiveness in humans is linked, at least in part, to differences in OspC affinity for plasminogen.
ISSN:1286-4579
1769-714X
DOI:10.1016/j.micinf.2005.08.017