Toxoplasma gondii Sequesters Lysosomes from Mammalian Hosts in the Vacuolar Space

The intracellular compartment harboring Toxoplasma gondii satisfies the parasite's nutritional needs for rapid growth in mammalian cells. We demonstrate that the parasitophorous vacuole (PV) of T. gondii accumulates material coming from the host mammalian cell via the exploitation of the host e...

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Bibliographic Details
Published in:Cell 2006-04, Vol.125 (2), p.261-274
Main Authors: Coppens, Isabelle, Dunn, Joe Dan, Romano, Julia D., Pypaert, Marc, Zhang, Hui, Boothroyd, John C., Joiner, Keith A.
Format: Article
Language:English
Subjects:
Animals
Antigens, Protozoan - metabolism
Cell Line
Cell Membrane - metabolism
Cercopithecus aethiops
Endosomes - metabolism
Endosomes - ultrastructure
Lipids
Liposomes - metabolism
Lysosomes - metabolism
Lysosomes - ultrastructure
Microtubules - metabolism
Protozoan Proteins - metabolism
Toxoplasma - cytology
Toxoplasma - metabolism
Toxoplasma gondii
Vacuoles - metabolism
Vacuoles - ultrastructure
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Summary:The intracellular compartment harboring Toxoplasma gondii satisfies the parasite's nutritional needs for rapid growth in mammalian cells. We demonstrate that the parasitophorous vacuole (PV) of T. gondii accumulates material coming from the host mammalian cell via the exploitation of the host endo-lysosomal system. The parasite actively recruits host microtubules, resulting in selective attraction of endo-lysosomes to the PV. Microtubule-based invaginations of the PV membrane serve as conduits for the delivery of host endo-lysosomes within the PV. These tubular conduits are decorated by a parasite coat, including the tubulogenic protein GRA7, which acts like a garrote that sequesters host endocytic organelles in the vacuolar space. These data define an unanticipated process allowing the parasite intimate and concentrated access to a diverse range of low molecular weight components produced by the endo-lysosomal system. More generally, they identify a unique mechanism for unidirectional transport and sequestration of host organelles.
ISSN:0092-8674
1097-4172
DOI:10.1016/j.cell.2006.01.056