Gastrodia elata Blume and an Active Component, p-Hydroxybenzyl Alcohol Reduce Focal Ischemic Brain Injury through Antioxidant Related Gene Expressions

Ischaemic stroke is a leading cause of death and long-lasting disability. Gastrodia elata blume (GEB) is a Chinese herb that is widely used to treat convulsive disorders, such as epilepsy, and p-hydroxybenzyl alcohol (HBA) is the active ingredient in GEB. The present study was conducted to evaluate...

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Published in:Biological & Pharmaceutical Bulletin 2005, Vol.28(6), pp.1016-1020
Main Authors: Yu, Seong Jin, Kim, Ju Ran, Lee, Chae Kwan, Han, Jeong Eun, Lee, Jae Hyun, Kim, Hye-Sook, Hong, Jeong Hwa, Kang, Sung Goo
Format: Article
Language:English
Subjects:
1-Cys peroxiredoxin
Animals
Antioxidants - metabolism
Benzyl Alcohols - isolation & purification
Benzyl Alcohols - pharmacology
Benzyl Alcohols - therapeutic use
Brain Ischemia - drug therapy
Brain Ischemia - metabolism
Brain Ischemia - pathology
Gastrodia
Gastrodia elata blume
Gene Expression Regulation - drug effects
Gene Expression Regulation - physiology
ischemia
Male
p-hydroxybenzyl alcohol
Plant Extracts - isolation & purification
Plant Extracts - pharmacokinetics
Plant Extracts - therapeutic use
protein disulfide isomerase
Rats
Rats, Sprague-Dawley
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creator Yu, Seong Jin
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description Ischaemic stroke is a leading cause of death and long-lasting disability. Gastrodia elata blume (GEB) is a Chinese herb that is widely used to treat convulsive disorders, such as epilepsy, and p-hydroxybenzyl alcohol (HBA) is the active ingredient in GEB. The present study was conducted to evaluate the effects of GEB and HBA on the brain damage and transcriptional levels of Protein disulfide isomerase (PDI) and 1-Cys peroxiredoxin (1-Cys Prx) genes known to play a role in antioxidant systems after transient focal ischemia in the rat brain. Focal ischemia was induced in rats by middle cerebral artery occlusion (MCAO). All animals underwent ischemia for 1 h, followed by 24 h of reperfusion. Coronal brain slices were stained with 2,3,5-triphenyltetrazolium chloride or total RNA was extracted for the analysis of gene expression. Histopathologic analysis revealed a significant (p
doi_str_mv 10.1248/bpb.28.1016
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Gastrodia elata blume (GEB) is a Chinese herb that is widely used to treat convulsive disorders, such as epilepsy, and p-hydroxybenzyl alcohol (HBA) is the active ingredient in GEB. The present study was conducted to evaluate the effects of GEB and HBA on the brain damage and transcriptional levels of Protein disulfide isomerase (PDI) and 1-Cys peroxiredoxin (1-Cys Prx) genes known to play a role in antioxidant systems after transient focal ischemia in the rat brain. Focal ischemia was induced in rats by middle cerebral artery occlusion (MCAO). All animals underwent ischemia for 1 h, followed by 24 h of reperfusion. Coronal brain slices were stained with 2,3,5-triphenyltetrazolium chloride or total RNA was extracted for the analysis of gene expression. Histopathologic analysis revealed a significant (p&lt;0.05) decrease in infarct size in the ipsilateral brain with GEB extracts or HBA. Moreover, the levels of PDI and 1-Cys Prx transcription were significantly increased in the GEB extract- or HBA-treated group compared with the untreated group (p&lt;0.05). This study therefore indicated that GEB and HBA provide neuroprotection by preventing brain damage through the increased expression of genes encoding antioxidant proteins after transient focal cerebral ischemia and may be effective as neuroprotective agents at the cellular and molecular levels in the brain.</description><identifier>ISSN: 0918-6158</identifier><identifier>EISSN: 1347-5215</identifier><identifier>DOI: 10.1248/bpb.28.1016</identifier><identifier>PMID: 15930737</identifier><language>eng</language><publisher>Japan: The Pharmaceutical Society of Japan</publisher><subject>1-Cys peroxiredoxin ; Animals ; Antioxidants - metabolism ; Benzyl Alcohols - isolation &amp; purification ; Benzyl Alcohols - pharmacology ; Benzyl Alcohols - therapeutic use ; Brain Ischemia - drug therapy ; Brain Ischemia - metabolism ; Brain Ischemia - pathology ; Gastrodia ; Gastrodia elata blume ; Gene Expression Regulation - drug effects ; Gene Expression Regulation - physiology ; ischemia ; Male ; p-hydroxybenzyl alcohol ; Plant Extracts - isolation &amp; purification ; Plant Extracts - pharmacokinetics ; Plant Extracts - therapeutic use ; protein disulfide isomerase ; Rats ; Rats, Sprague-Dawley</subject><ispartof>Biological and Pharmaceutical Bulletin, 2005, Vol.28(6), pp.1016-1020</ispartof><rights>2005 The Pharmaceutical Society of Japan</rights><rights>Copyright Japan Science and Technology Agency 2005</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c689t-24e2974d407cae9e990a16498e5dee0314bbf477bfbb074866f31864b0acc5a73</citedby><cites>FETCH-LOGICAL-c689t-24e2974d407cae9e990a16498e5dee0314bbf477bfbb074866f31864b0acc5a73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15930737$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yu, Seong Jin</creatorcontrib><creatorcontrib>Kim, Ju Ran</creatorcontrib><creatorcontrib>Lee, Chae Kwan</creatorcontrib><creatorcontrib>Han, Jeong Eun</creatorcontrib><creatorcontrib>Lee, Jae Hyun</creatorcontrib><creatorcontrib>Kim, Hye-Sook</creatorcontrib><creatorcontrib>Hong, Jeong Hwa</creatorcontrib><creatorcontrib>Kang, Sung Goo</creatorcontrib><creatorcontrib>aSchool of Biotechnology and Biomedical Science</creatorcontrib><creatorcontrib>Inje University</creatorcontrib><creatorcontrib>Institute of Basic Science</creatorcontrib><creatorcontrib>bInstitute of Industrial Medicine</creatorcontrib><creatorcontrib>dFaculty of Pharmaceutical Sciences</creatorcontrib><creatorcontrib>cDepartment of Food Science and Engineering</creatorcontrib><creatorcontrib>Okayama University</creatorcontrib><title>Gastrodia elata Blume and an Active Component, p-Hydroxybenzyl Alcohol Reduce Focal Ischemic Brain Injury through Antioxidant Related Gene Expressions</title><title>Biological &amp; Pharmaceutical Bulletin</title><addtitle>Biol Pharm Bull</addtitle><description>Ischaemic stroke is a leading cause of death and long-lasting disability. Gastrodia elata blume (GEB) is a Chinese herb that is widely used to treat convulsive disorders, such as epilepsy, and p-hydroxybenzyl alcohol (HBA) is the active ingredient in GEB. The present study was conducted to evaluate the effects of GEB and HBA on the brain damage and transcriptional levels of Protein disulfide isomerase (PDI) and 1-Cys peroxiredoxin (1-Cys Prx) genes known to play a role in antioxidant systems after transient focal ischemia in the rat brain. Focal ischemia was induced in rats by middle cerebral artery occlusion (MCAO). All animals underwent ischemia for 1 h, followed by 24 h of reperfusion. Coronal brain slices were stained with 2,3,5-triphenyltetrazolium chloride or total RNA was extracted for the analysis of gene expression. Histopathologic analysis revealed a significant (p&lt;0.05) decrease in infarct size in the ipsilateral brain with GEB extracts or HBA. Moreover, the levels of PDI and 1-Cys Prx transcription were significantly increased in the GEB extract- or HBA-treated group compared with the untreated group (p&lt;0.05). 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Moreover, the levels of PDI and 1-Cys Prx transcription were significantly increased in the GEB extract- or HBA-treated group compared with the untreated group (p&lt;0.05). This study therefore indicated that GEB and HBA provide neuroprotection by preventing brain damage through the increased expression of genes encoding antioxidant proteins after transient focal cerebral ischemia and may be effective as neuroprotective agents at the cellular and molecular levels in the brain.</abstract><cop>Japan</cop><pub>The Pharmaceutical Society of Japan</pub><pmid>15930737</pmid><doi>10.1248/bpb.28.1016</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record>
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subjects 1-Cys peroxiredoxin
Animals
Antioxidants - metabolism
Benzyl Alcohols - isolation & purification
Benzyl Alcohols - pharmacology
Benzyl Alcohols - therapeutic use
Brain Ischemia - drug therapy
Brain Ischemia - metabolism
Brain Ischemia - pathology
Gastrodia
Gastrodia elata blume
Gene Expression Regulation - drug effects
Gene Expression Regulation - physiology
ischemia
Male
p-hydroxybenzyl alcohol
Plant Extracts - isolation & purification
Plant Extracts - pharmacokinetics
Plant Extracts - therapeutic use
protein disulfide isomerase
Rats
Rats, Sprague-Dawley
title Gastrodia elata Blume and an Active Component, p-Hydroxybenzyl Alcohol Reduce Focal Ischemic Brain Injury through Antioxidant Related Gene Expressions
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