Effect of enzymatic and NaOCl treatments on acrylic roughness and on biofilm accumulation

• Published in: Journal of oral rehabilitation 2006-05, Vol.33 (5), p.356-362
• Main Authors: LIMA, E. M. C. X., MOURA, J. S., DEL BEL CURY, A. A., GARCIA, R. C. M. R., CURY, J. A.
• Format: Article
• Language: English
• Subjects: acrylic resin; Acrylic Resins - chemistry; Adult; biofilm; Biofilms - drug effects; Cross-Over Studies; Dentistry; denture cleansers; Denture Cleansers - pharmacology; Equipment Contamination - prevention & control; Humans; Orthodontic Appliances, Removable - microbiology; Single-Blind Method; Sodium Hypochlorite - pharmacology; Surface Properties - drug effects; surface roughness
• ISSN: 0305-182X; 1365-2842
• DOI: 10.1111/j.1365-2842.2005.01564.x

summary  As denture cleansers should be able to reduce biofilm accumulation without changing the acrylic resin surface structure, this study evaluated the effect of denture cleansers on surface roughness of acrylic resin and on biofilm accumulation. It was conducted a crossover study of three phases of 4 days each and 13 volunteers wore palatal appliances containing four specimens of acrylic resin of known surface roughness which were extra‐orally submitted once a day to three groups of separated treatments: (i) negative control, (ii) enzymatic commercial solution (Ortoform) or (iii) 0·5% sodium hypochlorite (NaOCl).The appliances were immersed eight times a day in a 20% sucrose solution to enhance biofilm formation. On morning of the fifth day of each phase, the amount of biofilm formed on the specimens was estimated by the amount of protein extracted with alkali and the surface roughness of the specimens was again measured. New palatal appliances with new specimens were worn by the volunteers in the following phases, but the treatments were changed. The roughness of the resin increased after the treatments (P