New Methods for the Generation of Carbohydrate Arrays on Glass Slides and Their Evaluation

Glycosides, having spacers functionalized with an aldehyde or a carboxylic group, were immobilized through reductive amination or amidation, respectively, onto amino-functionalized glass slides. Hybridization experiments with lectins exhibited very little nonspecific protein binding, hence precludin...

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Bibliographic Details
Published in:Chembiochem : a European journal of chemical biology 2005-06, Vol.6 (6), p.1007-1015
Main Authors: Biskup, Moritz B, Müller, Jan U, Weingart, Ralf, Schmidt, Richard R
Format: Article
Language:English
Subjects:
Amides - chemistry
Amination
arrays
Carbohydrate Metabolism
carbohydrates
Carbohydrates - chemistry
Coated Materials, Biocompatible
covalent ligation
Fluorescent Dyes - chemistry
fluorescent probes
Glass - chemistry
Glycosides - chemical synthesis
Glycosylation
lectins
Lectins - chemistry
Lectins - metabolism
Microchemistry - methods
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Summary:Glycosides, having spacers functionalized with an aldehyde or a carboxylic group, were immobilized through reductive amination or amidation, respectively, onto amino-functionalized glass slides. Hybridization experiments with lectins exhibited very little nonspecific protein binding, hence precluding the necessity for the blocking of unreacted functional groups on the glass slide. The covalency and the concentration dependency of the sugar ligation to the glass slide were demonstrated; the reversibility and the selectivity of lectin-carbohydrate interactions were shown.
ISSN:1439-4227
1439-7633
DOI:10.1002/cbic.200400300