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New Methods for the Generation of Carbohydrate Arrays on Glass Slides and Their Evaluation

Glycosides, having spacers functionalized with an aldehyde or a carboxylic group, were immobilized through reductive amination or amidation, respectively, onto amino-functionalized glass slides. Hybridization experiments with lectins exhibited very little nonspecific protein binding, hence precludin...

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Published in:	Chembiochem : a European journal of chemical biology 2005-06, Vol.6 (6), p.1007-1015
Main Authors:	Biskup, Moritz B, Müller, Jan U, Weingart, Ralf, Schmidt, Richard R
Format:	Article
Language:	English
Subjects:	Amides - chemistry Amination arrays Carbohydrate Metabolism carbohydrates Carbohydrates - chemistry Coated Materials, Biocompatible covalent ligation Fluorescent Dyes - chemistry fluorescent probes Glass - chemistry Glycosides - chemical synthesis Glycosylation lectins Lectins - chemistry Lectins - metabolism Microchemistry - methods
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creator	Biskup, Moritz B Müller, Jan U Weingart, Ralf Schmidt, Richard R
description	Glycosides, having spacers functionalized with an aldehyde or a carboxylic group, were immobilized through reductive amination or amidation, respectively, onto amino-functionalized glass slides. Hybridization experiments with lectins exhibited very little nonspecific protein binding, hence precluding the necessity for the blocking of unreacted functional groups on the glass slide. The covalency and the concentration dependency of the sugar ligation to the glass slide were demonstrated; the reversibility and the selectivity of lectin-carbohydrate interactions were shown.
doi_str_mv	10.1002/cbic.200400300
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subjects	Amides - chemistry Amination arrays Carbohydrate Metabolism carbohydrates Carbohydrates - chemistry Coated Materials, Biocompatible covalent ligation Fluorescent Dyes - chemistry fluorescent probes Glass - chemistry Glycosides - chemical synthesis Glycosylation lectins Lectins - chemistry Lectins - metabolism Microchemistry - methods
title	New Methods for the Generation of Carbohydrate Arrays on Glass Slides and Their Evaluation
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