Molecular characterization of the rpoB gene in Brucella species: new potential molecular markers for genotyping

The rpoB gene encoding the β subunit of the DNA-dependent RNA polymerase was molecularly characterized by PCR amplification and DNA sequencing in 26 Brucella reference strains by using primers selected according to the B. melitensis 16M rpoB published sequence. Comparison of the rpoB nucleotide sequ...

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Bibliographic Details
Published in:Microbes and infection 2006-03, Vol.8 (3), p.860-865
Main Authors: Marianelli, Cinzia, Ciuchini, Franco, Tarantino, Michela, Pasquali, Paolo, Adone, Rosanna
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Anti-Bacterial Agents - pharmacology
Bacterial Proteins - chemistry
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Bacteriology
Base Sequence
Biological and medical sciences
Brucella
Brucella - drug effects
Brucella - genetics
DNA sequencing
Drug Resistance, Bacterial
Fundamental and applied biological sciences. Psychology
Genes, Bacterial
Genetic Markers
Genotype
Genotyping
Microbiology
Miscellaneous
Molecular Sequence Data
Rifampin - pharmacology
rpoB gene
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Summary:The rpoB gene encoding the β subunit of the DNA-dependent RNA polymerase was molecularly characterized by PCR amplification and DNA sequencing in 26 Brucella reference strains by using primers selected according to the B. melitensis 16M rpoB published sequence. Comparison of the rpoB nucleotide sequence of all Brucella strains analysed revealed specific nucleotide variations associated with different Brucella species and biovars. 17 rpoB alleles were recognized and new Brucella typing is proposed. Our results suggest that the rpoB gene polymorphism can be used to identify all Brucella species and most of the biovars, offering an improvement over conventional typing methods.
ISSN:1286-4579
1769-714X
DOI:10.1016/j.micinf.2005.10.008