Characterization of the polymorphic repeat sequence within the rDNA IGS of Cannabis sativa

The rDNA intergenic spacer (IGS) structure of Cannabis sativa contains six variable repeat motifs within a locus spanning 1387 base pairs. The degree of variation of the first three motifs was examined using 77 samples from cannabis samples. The samples originated from five seizures in Taiwan and se...

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Bibliographic Details
Published in:Forensic science international 2005-08, Vol.152 (1), p.23-28
Main Authors: Hsieh, Hsing-Mei, Liu, Chia-Ling, Tsai, Li-Chin, Hou, Rur-Jyun, Liu, Kuo-Lan, Linacre, Adrian, Lee, James Chun-I
Format: Article
Language:English
Subjects:
Analysis
Cannabis
Cannabis - genetics
Cannabis sativa
Deoxyribonucleic acid
DNA
DNA, Intergenic - analysis
DNA, Plant - analysis
Forensic Medicine
Forensic sciences
Genetic aspects
Genetic polymorphisms
Genomes
Genomics
Microbiology
Nucleotide sequence
Polymerase Chain Reaction
Polymorphism
Polymorphism, Genetic
rDNA IGS
Repeat sequence
Ribosomal DNA
Seeds
Sequence Analysis, DNA
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Summary:The rDNA intergenic spacer (IGS) structure of Cannabis sativa contains six variable repeat motifs within a locus spanning 1387 base pairs. The degree of variation of the first three motifs was examined using 77 samples from cannabis samples. The samples originated from five seizures in Taiwan and seed stocks from six different countries. The results showed that there were four types of sequences producing PCR products at either 255, 260, 264 or 265 base pairs. The data obtained indicates that this region of rDNA IGS exhibits a degree of polymorphism that while insufficient by itself can be added to a multiplex with other cannabis STR loci.
ISSN:0379-0738
1872-6283
DOI:10.1016/j.forsciint.2005.02.022