Increased expression of TGF-beta1 reduces tumor growth of human U-87 Glioblastoma Cells in vivo

The role that transforming growth factor beta1 (TGF-beta1) plays in influencing growth of glioma cells is somewhat controversial. To further understand the potential growth-regulatory effects of TGF-beta1,we constructed an animal astroglial tumor model by injecting either wild-type or virally transd...

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Published in:Cancer immunology, immunotherapy : CII immunotherapy : CII, 2006-08, Vol.55 (8), p.918-927
Main Authors: Pan, Jen-Jung, Chang, Wei-Jen, Barone, Tara A, Plunkett, Robert J, Ostrow, Peter T, Greenberg, Steven J
Format: Article
Language:English
Subjects:
Animals
Biomarkers, Tumor - analysis
Blotting, Northern
Blotting, Western
Brain Neoplasms - metabolism
Brain Neoplasms - pathology
Cell Line, Tumor
Cell Proliferation
Disease Models, Animal
DNA Fragmentation
Glioblastoma - metabolism
Glioblastoma - pathology
Humans
Immunohistochemistry
In Situ Nick-End Labeling
Rats
Rats, Nude
Receptors, Transforming Growth Factor beta - biosynthesis
Transduction, Genetic
Transforming Growth Factor beta1 - biosynthesis
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Summary:The role that transforming growth factor beta1 (TGF-beta1) plays in influencing growth of glioma cells is somewhat controversial. To further understand the potential growth-regulatory effects of TGF-beta1,we constructed an animal astroglial tumor model by injecting either wild-type or virally transduced human U-87 glioblastoma cells into nude rat brains. Wild type U-87 cells produced very low amounts of TGF-beta1 and were highly tumorigenic. In contrast, U-87 cells transduced to express high levels of TGF-beta1 showed reduced tumor size in vivo, in a dose-dependent manner. This reduction in tumor size was not due to either decreased vascularity or increased apoptosis. To test whether TGF-beta1 overproduction inhibited tumor growth through an autocrine mechanism, the highest TGF-beta1 producing cells were then double transduced with a vector expressing the kinase-truncated type II TGF-beta receptor. Cells expressing high levels of truncated TGF-beta receptor were less sensitive to TGF-beta1 mediated growth inhibition in vitro and produced more aggressive tumors in vivo. The data suggest that the degree of tumorigenicity of the U-87 high-grade glioblastoma cell line may be associated with correspondingly low level of production of TGF-beta1. These results also would tend to support the possibility that TGF-beta1 may be useful in treating some high-grade gliomas.
ISSN:0340-7004