Procyanidin Fractions from Pine (Pinus pinaster) Bark:  Radical Scavenging Power in Solution, Antioxidant Activity in Emulsion, and Antiproliferative Effect in Melanoma Cells

Pine (Pinus pinaster) bark is a rich source of procyanidin oligomers. From a total polyphenolic extract, we have generated fractions of different procyanidin composition. The mixtures, devoid of gallate esters, were active as free radical scavengers against ABTS•+, DPPH, and HNTTM. Pine bark fractio...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2005-06, Vol.53 (12), p.4728-4735
Main Authors: Touriño, Sonia, Selga, Ariadna, Jiménez, Aurora, Juliá, Lluís, Lozano, Carles, Lizárraga, Daneida, Cascante, Marta, Torres, Josep Lluís
Format: Article
Language:English
Subjects:
Antioxidants - pharmacology
Cell Division - drug effects
Emulsions
Free Radical Scavengers - pharmacology
Humans
Melanoma - pathology
Pinus - chemistry
Plant Bark - chemistry
Proanthocyanidins - analysis
Proanthocyanidins - pharmacology
Solutions
Tumor Cells, Cultured
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Summary:Pine (Pinus pinaster) bark is a rich source of procyanidin oligomers. From a total polyphenolic extract, we have generated fractions of different procyanidin composition. The mixtures, devoid of gallate esters, were active as free radical scavengers against ABTS•+, DPPH, and HNTTM. Pine bark fractions were tested for antioxidant activity in solution (hydrogen donation and electron transfer) and emulsion (inhibition of lipid peroxidation) and compared with their galloylated counterparts from grape origin. While galloylation clearly influenced the free radical scavenging efficiency in solution, it did not seem to play a determinant role in protection against lipid peroxidation in emulsion. The fractions were very mild inhibitors of cell proliferation. Because gallate esters appear to interfere with crucial cell functions, gallate free pine procyanidins may be the innocuous chemopreventative agents of choice for many applications in food and skin protection. Keywords: Pinus pinaster; polyphenols; procyanidins; catechins; antioxidants; free radical scavenging activity; emulsions; cell proliferation
ISSN:0021-8561
1520-5118
DOI:10.1021/jf050262q