Effect of long-term culture on the expression of antigens and adhesion molecule in single porcine pancreatic endocrine cells

• Published in: Xenotransplantation (Københaven) 2005-07, Vol.12 (4), p.327-332
• Main Authors: Edamura, Kazuya, Nasu, Koko, Iwami, Yukiko, Nishimura, Ryohei, Ogawa, Hiroyuki, Sasaki, Nobuo, Ohgawara, Hisako
• Format: Article
• Language: English
• Subjects: Animals; Antigens - genetics; Cell Adhesion Molecules - genetics; culture; Culture Techniques; Endocrine System - cytology; Galα1-3Gal; Gene Expression Regulation; ICAM-1; Pancreas - cytology; porcine pancreatic endocrine cell; RNA, Messenger - analysis; RNA, Messenger - genetics; SLA class II; Swine; Time Factors
• ISSN: 0908-665X; 1399-3089
• DOI: 10.1111/j.1399-3089.2005.00232.x

:  Background:  Changes in the expression of galactose (Gal) α1‐3Gal, swine lymphocyte antigen (SLA) class II and intracellular adhesion molecule (ICAM)‐1 of single porcine pancreatic endocrine (PE) cells during the culture period were investigated. Methods:  Cultured porcine PE‐cells were fixed in 10% buffered formalin for histological evaluation. At 1, 3, 6, 9 and 12 weeks of culture, mRNA was obtained from porcine PE‐cells so that the expression of SLA class II and ICAM‐1 genes could be examined by reverse transcriptase‐polymerase chain reaction. Results:  The rates of Galα1‐3Gal and SLA class II‐positive cells did not decrease during the culture period, but the rates of Galα1‐3Gal and SLA class II strongly positive cells significantly decreased. ICAM‐1‐positive cells were scarcely observed during the culture period. SLA class II and ICAM‐1 mRNAs were detected at 1 and 3 weeks of culture, but were not detected after 6 weeks of culture. Conclusions:  These results suggest that partial reduction in the expression of these antigens could be obtained by a long‐term culture.