Impact of moisture on in vitro germination of Metarhizium anisopliae and Beauveria bassiana and their activity on Triatoma infestans

The in vitro germination of 11 Metarhizium anisopliae and 11 Beauveria bassiana isolates originating from substrates collected in rural peridomestic areas in Central Brazil where triatomines are common was tested. Conidia completed germination up to 24 h after exposure to water activity of >0.99...

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Bibliographic Details
Published in:Mycological research 2006-04, Vol.110 (4), p.485-492
Main Authors: Lazzarini, Gustavo Maximiano Junqueira, Rocha, Luiz Fernando Nunes, Luz, Christian
Format: Article
Language:English
Subjects:
Animals
Ascomycota - growth & development
Beauveria bassiana
Biocontrol
biological control
biological control agents
Chagas disease
entomopathogenic fungi
Entomopathogens
human health
Humidity
insect control
Insect pathology
insect vectors
Insect Vectors - microbiology
Metarhizium anisopliae
Pest Control, Biological - methods
relative humidity
spore germination
Statistics, Nonparametric
Triatoma - microbiology
Triatoma infestans
Triatomines
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Summary:The in vitro germination of 11 Metarhizium anisopliae and 11 Beauveria bassiana isolates originating from substrates collected in rural peridomestic areas in Central Brazil where triatomines are common was tested. Conidia completed germination up to 24 h after exposure to water activity of >0.99 a w in all isolates tested. At lower 0.93 a w germination was delayed but conidia of most isolates germinated at high rates (>98 %) within 216 h of incubation. Activities of 2 M. anisopliae and 2 B. bassiana isolates with different patterns of germination at 0.93 a w were tested in Triatoma infestans third instar nymphs. There was no relationship between germination kinetics in vitro at 0.93 a w and their activity in vivo at 98, 75 and 43 % relative humidity (rh). Isolates with accelerated germination at 0.93 a w were not more virulent at 75 and 43 % rh compared with isolates with retarded or no germination. Highest mortalities were observed at 98 % rh, and they did not exceed 25 % after 25 d incubation at lower 75 and 43 % rh. Isolates that originated from a region with an extensive annual arid period showed no adaptation to lower humidity in their activity against T. infestans.
ISSN:0953-7562
1469-8102
DOI:10.1016/j.mycres.2005.12.001