Expression of the 5-HT2A serotoninergic receptor in human placenta and choriocarcinoma cells : Mitogenic implications of serotonin

Serotonin (5-hydroxytryptamine, 5-HT) has diverse physiological functions and acts as a mitogen in a variety of cell types, including bovine placental cells. It exerts its mitogenic effect by interacting with a wide range of 5-HT receptor types. Previous studies have reported the presence of 5-HT(2)...

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Bibliographic Details
Published in:Placenta (Eastbourne) 2005-07, Vol.26 (6), p.484-490
Main Authors: SONIER, B, LAVIGNE, C, ARSENEAULT, M, OUELLETTE, R, VAILLANCOURT, C
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cell Division - physiology
Cell Line, Tumor
Choriocarcinoma - pathology
Choriocarcinoma - physiopathology
Embryology: invertebrates and vertebrates. Teratology
Female
Fetal membranes
Fluorescent Antibody Technique
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Neoplastic - physiology
General aspects. Development. Fetal membranes
Humans
Mitogens - physiology
Placenta - cytology
Placenta - physiology
Pregnancy
Receptor, Serotonin, 5-HT2A - genetics
Receptor, Serotonin, 5-HT2A - metabolism
RNA, Messenger - analysis
Serotonin - physiology
Uterine Neoplasms - pathology
Uterine Neoplasms - physiopathology
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Summary:Serotonin (5-hydroxytryptamine, 5-HT) has diverse physiological functions and acts as a mitogen in a variety of cell types, including bovine placental cells. It exerts its mitogenic effect by interacting with a wide range of 5-HT receptor types. Previous studies have reported the presence of 5-HT(2) binding sites in human placental trophoblastic cells, but this has never been confirmed at the molecular level. In this study, we demonstrated that the 5-HT(2A) receptor subtype is fully expressed in the human choriocarcinoma cell lines JEG-3 and BeWo as well as in normal human placental tissue. DNA sequencing has confirmed that the 5-HT(2A) receptor present in these cell lines and tissues is identical to the human 5-HT(2A) receptor found in platelets and in the cerebral cortex. This receptor was localized by immunofluorescence on the plasma membrane, in JEG-3 and BeWo cells. Furthermore, MTT proliferation assays revealed a positive effect of 5-HT on the proliferation of JEG-3 and BeWo cells. These results suggest that 5-HT constitutes a potent mitogen for neoplastic placental cells.
ISSN:0143-4004
1532-3102
DOI:10.1016/j.placenta.2004.08.003