Models for estimating parameters of neutral detergent fiber digestion by ruminal microorganisms

• Published in: Journal of animal science 2005-07, Vol.83 (7), p.1591-1601
• Main Authors: Ellis, W. C, Mahlooji, M, Matis, J. H
• Format: Article
• Language: English
• Subjects: Animal Nutritional Physiological Phenomena; Animal productions; Animals; beef cattle; Biological and medical sciences; Cattle; corn silage; Cynodon - metabolism; Dietary fiber; Dietary Fiber - classification; Dietary Fiber - metabolism; digestion; Digestion - physiology; Digestive system; Fundamental and applied biological sciences. Psychology; hay; in vitro digestion; Male; mastication; Microorganisms; Models, Biological; neutral detergent fiber; Parameter estimation; particle size; Rumen - metabolism; Rumen - microbiology; rumen fluids; rumen microorganisms; Ruminants - metabolism; simulation models; steers; Terrestrial animal productions; Time Factors; Tissues; Vertebrates; Zea mays - metabolism
• ISSN: 0021-8812; 1525-3163
• DOI: 10.2527/2005.8371591x

Model assumptions included number of concurrently degrading entities (or pools) and expected distributions of undegraded NDF. Degradation processes modeled included a single pool with ruminal age-constant rates (exponential distribution), a single pool with a ruminal age-dependent rate, two pools with age-constant rates, two pools with age-dependent and age-constant rates, and a continuum of pools with a gamma distribution of age-constant rates. Various sizes of ingestively masticated fragments of bermudagrass hay or corn silage were obtained via wet sieving of esophageal masticate and incubated in vitro with ruminal fluid for 0 h, every 6 h up to 48 h, and every 12 h up to 168 h. Models assuming a single pool of age-constant or age-dependent rates had larger mean residual mean squares (P < 0.05) than did the gamma mixture model or the two-pool models. Degradation rates estimated by the gamma mixture model indicated distribution of rates ranging from near exponential, age-constant distribution to a near normal bell-shaped distribution of age-constant rates for different datasets. Superior fit by the two-pool models in most datasets (83%) indicated that having two resolvable entities of potentially degradable NDF with different degradation rates was causal of a biphasic distribution of lifetimes. Increasing order of age-dependency modeled in the two-pool model improved fit and precision of estimation (standard error of estimate) for the limit parameters of time delay and indigestible NDF. Both the gamma mixture continuum of age-constant rate model and the two-pool, age-dependent models with a discrete time delay provided similar fit to data and flexibility for fitting data with lifetime distributions ranging from simple exponential to sigmodial. The two-pool, age-dependent and gamma-distributed, age-constant models were better in fitting the dominant biphasic lifetime distributions that occurred when the two pools of degrading entities were of similar size and in estimating the discrete time delay when strategic, quality data were available. Having fewer parameters (four), the gamma-distributed, age-constant model was superior when data quality was limited.