Mass spectrometric analysis of the Schistosoma mansoni tegumental sub-proteome

Schistosoma mansoni is a parasitic worm that lives in the blood vessels of its host. We mapped the S. mansoni tegumental outer-surface structure proteome by 1D SDS-PAGE and LC-MS/MS and an EST-database from the ongoing genome-sequencing project. We identified 740 proteins of which 43 were tegument-s...

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Bibliographic Details
Published in:Journal of proteome research 2005-05, Vol.4 (3), p.958-966
Main Authors: van Balkom, Bas W M, van Gestel, Renske A, Brouwers, Jos F H M, Krijgsveld, Jeroen, Tielens, Aloysius G M, Heck, Albert J R, van Hellemond, Jaap J
Format: Article
Language:English
Subjects:
Animals
Chromatography, High Pressure Liquid
Computational Biology
Electrophoresis, Polyacrylamide Gel
Helminth Proteins - analysis
Helminth Proteins - physiology
Mass Spectrometry
Proteome - analysis
Proteomics - methods
Schistosoma mansoni - chemistry
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Summary:Schistosoma mansoni is a parasitic worm that lives in the blood vessels of its host. We mapped the S. mansoni tegumental outer-surface structure proteome by 1D SDS-PAGE and LC-MS/MS and an EST-database from the ongoing genome-sequencing project. We identified 740 proteins of which 43 were tegument-specific. Many of these proteins show no homology to any nonschistosomal protein, demonstrating that the schistosomal outer-surface comprises specific and unique proteins, likely to be critical for parasite survival.
ISSN:1535-3893
DOI:10.1021/pr050036w