Direct Protein Detection from Biological Media through Electrospray-Assisted Laser Desorption Ionization/Mass Spectrometry

We report here using a novel technologyelectrospray-assisted laser desorption ionization (ELDI)/mass spectrometryfor the rapid and sensitive detection of the major proteins that exist in dried biological fluids (e.g., blood, tears, saliva, serum), bacterial cultures, and tissues (e.g., porcine liv...

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Bibliographic Details
Published in:Journal of proteome research 2006-05, Vol.5 (5), p.1107-1116
Main Authors: Huang, Min-Zong, Hsu, Hsiu-Jung, Lee, Jen-Yih, Jeng, Jingyueh, Shiea, Jentaie
Format: Article
Language:English
Subjects:
Animals
Bacterial Proteins - analysis
Humans
Liver - chemistry
Myocardium - chemistry
Proteins - analysis
Proteins - chemistry
Rabbits
Rats
Specimen Handling - methods
Spectrometry, Mass, Electrospray Ionization - methods
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
Swine
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Summary:We report here using a novel technologyelectrospray-assisted laser desorption ionization (ELDI)/mass spectrometryfor the rapid and sensitive detection of the major proteins that exist in dried biological fluids (e.g., blood, tears, saliva, serum), bacterial cultures, and tissues (e.g., porcine liver and heart) under ambient conditions. This technique required essentially no sample pretreatment. The proteins in the samples were desorbed using a pulsed nitrogen laser without the assistance of an organic matrix. The desorbed protein molecules were then post-ionized through their fusion into the charged solvent droplets produced from the electrospray of an acidic methanol solution; electrospray ionization (ESI) proceeded from the newly formed droplets to generate the ESI-like protein ions. This new ionization approach combines some of the features of electrospray ionization with those of matrix-assisted laser desorption ionization (MALDI), that is, sampling of a solid surface with spatial resolution, generating ESI-like mass spectra of the desorbed proteins, and operating under ambient conditions. Keywords: electrospray-assisted laser desorption/ionization • ELDI • post-ionization • laser desorption • two-step ESI • FD-ESI
ISSN:1535-3893
1535-3907
DOI:10.1021/pr050442f