Comparison of milk-ELISA and serum-ELISA for the diagnosis of Brucella melitensis infection in sheep

• Published in: Veterinary microbiology 2005-07, Vol.108 (3), p.305-311
• Main Authors: Chand, P., Rajpurohit, B.S., Malhotra, A.K., Poonia, J.S.
• Format: Article
• Language: English
• Subjects: agglutination tests; Agglutination Tests - veterinary; Animals; Antibodies, Bacterial - analysis; Antibodies, Bacterial - blood; antibody detection; Bacteriology; Biological and medical sciences; blood serum; Brucella melitensis; Brucella melitensis - isolation & purification; Brucellosis; Brucellosis - blood; Brucellosis - diagnosis; Brucellosis - microbiology; Brucellosis - veterinary; Diagnosis; diagnostic techniques; disease detection; enzyme-linked immunosorbent assay; Enzyme-Linked Immunosorbent Assay - methods; Enzyme-Linked Immunosorbent Assay - veterinary; ewe milk; ewes; Female; Fundamental and applied biological sciences. Psychology; isolation; Microbiology; Milk - microbiology; Milk-ELISA; Miscellaneous; Predictive Value of Tests; Sensitivity and Specificity; serodiagnosis; Serum-ELISA; Sheep; sheep diseases; Sheep Diseases - blood; Sheep Diseases - diagnosis; Sheep Diseases - microbiology
• ISSN: 0378-1135; 1873-2542
• DOI: 10.1016/j.vetmic.2005.04.006

Milk and blood samples from 704 lactating ewes were examined for the diagnosis of Brucella melitensis infection by milk-ELISA, serum-ELISA, RBPT, SAT and culture of milk. Of these ewes, 209 were from brucellosis free sheep flock, 443 from brucellosis infected sheep flock and 52 were from private sheep flocks of which status for brucellosis was not known. All the 209 ewes belonging to uninfected sheep flock were found negative in all the tests and of the remaining 495 ewes 105 were positive in serum-ELISA, 103 in milk-ELISA, 92 in RBPT, 85 in SAT, and B. melitensis biovar-1 was isolated from the milk of 29 ewes. Of the 105 serum-ELISA positive ewes, 99 were positive and 6 were negative in milk-ELISA, whereas of the 103 milk-ELISA positive ewes, 4 were negative in serum-ELISA. All together, 99 ewes were positive and 386 were negative in both the assays while 10 ewes yielded variable results. The specificity of milk-ELISA in brucellosis free flock was 100% and sensitivity and positive predictive value were 96.11% and 94.28%, respectively, in infected flocks. The Brucella antibody levels in milk and serum samples as determined by milk-ELISA and serum-ELISA were correlated significantly. The milk-ELISA for brucellosis appears to be an attractive alternative of serum-ELISA particularly in the lactating ewes.