Molecular cloning and expression analysis of the cell-wall invertase gene family in rice (Oryza sativa L.)

• Published in: Plant cell reports 2005-06, Vol.24 (4), p.225-236
• Main Authors: Cho, J.I, Lee, S.K, Ko, S, Kim, H.K, Jun, S.H, Lee, Y.H, Bhoo, S.H, Lee, K.W, An, G, Hahn, T.R
• Format: Article
• Language: English
• Subjects: amino acid sequences; Amino acids; Bacteria; beta-fructofuranosidase; beta-Fructofuranosidase - chemistry; beta-Fructofuranosidase - genetics; Biological and medical sciences; Biotechnology; Carbohydrates; Cell Wall - enzymology; Cell Wall - genetics; cell wall components; cell walls; chromosome mapping; Cloning; Cloning, Molecular; complementary DNA; Databases, Protein; DNA, Complementary - analysis; DNA, Complementary - genetics; Down-Regulation - drug effects; Down-Regulation - genetics; Energy Metabolism - genetics; Eukaryotic cell cultures; Flowers - enzymology; Flowers - genetics; Fundamental and applied biological sciences. Psychology; Gene Expression Profiling; gene expression regulation; Gene Expression Regulation, Plant - drug effects; Gene Expression Regulation, Plant - genetics; Genes, Plant - genetics; Genome, Plant; Immunity, Innate - genetics; Leaves; Magnaporthe grisea; Methods. Procedures. Technologies; Miscellaneous; Molecular Sequence Data; nucleotide sequences; Oryza - enzymology; Oryza - genetics; Oryza - growth & development; Oryza sativa; Phylogeny; Plant cells and fungal cells; plant pathogenic fungi; plant proteins; Plant Proteins - genetics; Plant Proteins - isolation & purification; Plant Proteins - metabolism; Rice; Seeds; Seeds - enzymology; Seeds - genetics; Sequence Homology, Amino Acid; Sucrose; Sucrose - metabolism; Sucrose - pharmacology
• ISSN: 0721-7714; 1432-203X
• DOI: 10.1007/s00299-004-0910-z

Cell-wall invertase (CIN) catalyzes the hydrolysis of sucrose into glucose and fructose for the supply of carbohydrates to sink organs via an apoplastic pathway. To study the CIN genes in rice (Oryza sativa L.), we isolated cDNA clones showing amino acid similarity to the plant cell wall invertase proteins from a search of rice sequence databases. Profile analyses revealed that the cloned genes are expressed in unique patterns in various organs. For example, transcripts of OsCIN1, OsCIN2, OsCIN4, and OsCIN7 were detected in immature seeds whereas OsCIN3 gene expression was flower-specific. Further transcript analysis of these genes expressed in developing seeds indicated that OsCIN1, OsCIN2, and OsCIN7 might play an important role involving sucrose partitioning to the embryo and endosperm. Sucrose, a substrate of CINs, induced the accumulation of OsCIN1 transcripts in excised leaves and OsCIN2 in immature seeds, while the level of OsCIN5 was significantly down-regulated in excised leaves treated with sucrose. Infecting the tissues with rice blast (Magnaporthe grisea) as a biotic stressor increased the expression of OsCIN1, OsCIN4, and OsCIN5, suggesting that these genes may participate in a switch in metabolism to resist pathogen invasion. These results demonstrate that OsCIN genes play diverse roles involving the regulation of metabolism, growth, development, and stress responses.