Achieving desired plant growth regulator levels in liquid plant tissue culture media that include activated carbon

This paper is part of a series considering the impact of activated carbon (AC) on the composition of plant tissue culture media. Using liquid culture media for initiation of Norway spruce embryogenic tissue and eight different ACs, we present a method for achieving target plant growth regulator (PGR...

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Bibliographic Details
Published in:Plant cell reports 2005-06, Vol.24 (4), p.201-208
Main Authors: Van Winkle, S.C, Pullman, G.S
Format: Article
Language:English
Subjects:
2,4-D
Activated carbon
benzyladenine
Biological and medical sciences
Biotechnology
Carbon
Cell Culture Techniques - methods
Charcoal - chemistry
Charcoal - pharmacology
Culture media
Culture Media - chemistry
Culture Media - pharmacology
Eukaryotic cell cultures
Fundamental and applied biological sciences. Psychology
Growth regulators
Linear Models
Methods. Procedures. Technologies
Miscellaneous
naphthaleneacetic acid
Picea - drug effects
Picea - embryology
Picea - growth & development
Picea abies
Pine trees
Plant cells and fungal cells
Plant growth
Plant Growth Regulators - pharmacology
plant growth substances
Plant tissues
Seeds - drug effects
Seeds - growth & development
somatic embryogenesis
somatic embryos
Surface area
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Summary:This paper is part of a series considering the impact of activated carbon (AC) on the composition of plant tissue culture media. Using liquid culture media for initiation of Norway spruce embryogenic tissue and eight different ACs, we present a method for achieving target plant growth regulator (PGR) levels in AC-containing medium based on sorption isotherms for individual PGRs. Linear relationships were found between PGR adsorption and specific BET (Brunauer, Emmett, Teller theory) surface area and specific total pore volume of AC. When using a new AC, this linear relationship allows one to achieve multiple PGR levels similar to historic levels through adjustment of the mass of AC based on its relative BET surface area or relative total pore volume. Target levels of PGRs and an initiation success similar to that in medium without AC were achieved with several different AC types when AC mass was adjusted on the basis of pore volume.
ISSN:0721-7714
1432-203X
DOI:10.1007/s00299-005-0931-2