Interactions of systemic immune response and local wound healing in different burn depths : An experimental study on rats

This study aimed to clarify the local and systemic immune responses at different burn depths. Thirty female Sprague-Dawley rats were divided into three groups: full-thickness (F), partial-thickness (P), and Sham (S). Burns were induced on three separate areas on the dorsums of rats. Serum levels of...

Full description

Saved in:
Bibliographic Details
Published in:Journal of burn care & research 2006-05, Vol.27 (3), p.357-366
Main Authors: EBRU SAKALLIOGLU, A, BASARAN, Ozgur, KARAKAYALI, Hamdi, HANDAN OZDEMIR, B, YUCEL, Muammer, ARAT, Zubeyde, HABERAL, Mehmet
Format: Article
Language:English
Subjects:
Animals
Antigens, CD - analysis
Biological and medical sciences
Burns
Burns - immunology
Burns - pathology
Burns - physiopathology
CD3 Complex - analysis
Dermatology
Female
HLA-DR Antigens
Inflammation - blood
Interferon-gamma - blood
Interleukin-1 - blood
Interleukin-10 - blood
Macrophages
Medical sciences
Rats
Rats, Sprague-Dawley
Time Factors
Traumas. Diseases due to physical agents
Tumor Necrosis Factor-alpha - analysis
Wound Healing - immunology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This study aimed to clarify the local and systemic immune responses at different burn depths. Thirty female Sprague-Dawley rats were divided into three groups: full-thickness (F), partial-thickness (P), and Sham (S). Burns were induced on three separate areas on the dorsums of rats. Serum levels of interferon (IFN)-gamma; tumor necrosis factor-alpha; interleukin (IL)-1, IL-6, and IL-10 were measured once in controls and 1 hour after burn, 48 hours after burn, and 7 days after burn in F and P groups. Neutrophils, CD68-positive macrophages, HLA-DR-positive cells, and CD3-positive lymphocytes were graded semiquantitatively, and the wounds were examined once in shams and at 1 hour after burn, 48 hours after burn, and 7 days after burn in F and P groups. IL-6 levels were highest in F group, followed by P group 1 hour after burn. IFN-gamma levels were higher in the F group; IL-1 levels were higher in F and P groups at 1 hour after burn. Local accumulation of macrophages was similar in F and P groups. Lymphocytes were denser in P group at 1 hour after burn, and neutrophils were denser in F group at 7 days after burn. We suggest that early elevations of IL-6 and IFN-gamma prolong inflammation in full-thickness burns. Modulation of proinflammatory cytokines may improve burn wound treatment.
ISSN:1559-047X
1559-0488
DOI:10.1097/01.bcr.0000216330.93056.06