Tilmicosin and tylosin have anti-inflammatory properties via modulation of COX-2 and iNOS gene expression and production of cytokines in LPS-induced macrophages and monocytes

Macrolides have been reported to modify the host immune and inflammatory responses both in vivo and in vitro. We examined the in vitro effect of the macrolides tilmicosin and tylosin, which are only used in the veterinary clinic, on the production of nitric oxide (NO), prostaglandin E 2 (PGE 2) and...

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Bibliographic Details
Published in:International journal of antimicrobial agents 2006-05, Vol.27 (5), p.431-438
Main Authors: Cao, Xing-Yuan, Dong, Mei, Shen, Jian-Zhong, Wu, Bei-Bei, Wu, Cong-Ming, Du, Xiang-Dang, Wang, Zhuo, Qi, Yi-Tao, Li, Bing-yu
Format: Article
Language:English
Subjects:
Animals
Antibiotics. Antiinfectious agents. Antiparasitic agents
Biological and medical sciences
Cyclooxygenase 2 - genetics
Cyclooxygenase 2 - metabolism
Cyclooxygenase 2 Inhibitors - pharmacology
Cyclooxygenase-2
Cytokines
Cytokines - metabolism
Dinoprostone - metabolism
Gene Expression Regulation, Enzymologic - drug effects
Inducible nitric oxide synthase
Lipopolysaccharides - pharmacology
Macrolides - pharmacology
Macrophage
Medical sciences
Mice
Monocytes - drug effects
Monocytes - metabolism
Nitric Oxide - metabolism
Nitric Oxide Synthase Type II - genetics
Nitric Oxide Synthase Type II - metabolism
PBMC
Pharmacology. Drug treatments
Tilmicosin
Tylosin - analogs & derivatives
Tylosin - pharmacology
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Summary:Macrolides have been reported to modify the host immune and inflammatory responses both in vivo and in vitro. We examined the in vitro effect of the macrolides tilmicosin and tylosin, which are only used in the veterinary clinic, on the production of nitric oxide (NO), prostaglandin E 2 (PGE 2) and cytokines by lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages and mouse peripheral blood mononuclear cells (PBMCs). Compared with 5 μg/mL, tilmicosin and tylosin concentrations of 10 μg/mL and 20 μg/mL significantly decreased the production of 6-keto-prostaglandin F 1α (6-keto-PGF 1α), PGE 2, NO, tumour necrosis factor-α (TNF-α), interleukin (IL)-1β and IL-6, and increased IL-10 production. Cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) gene expression were also significantly reduced. These results support the opinion that macrolides may exert an anti-inflammatory effect through modulating the synthesis of several mediators and cytokines involved in the inflammatory process.
ISSN:0924-8579
1872-7913
DOI:10.1016/j.ijantimicag.2005.12.010