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Activation of feeding-related neural circuitry after unilateral injections of muscimol into the nucleus accumbens shell
Chemical inhibition of neurons in the nucleus accumbens shell (AcbSh) elicits intense, behaviorally specific, feeding in satiated rats. We have demonstrated previously that this treatment activates a number of brain regions, most significantly the lateral hypothalamus (LH). This activation could be...
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Published in: | Brain research 2005-06, Vol.1048 (1), p.241-250 |
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description | Chemical inhibition of neurons in the nucleus accumbens shell (AcbSh) elicits intense, behaviorally specific, feeding in satiated rats. We have demonstrated previously that this treatment activates a number of brain regions, most significantly the lateral hypothalamus (LH). This activation could be elicited through a direct neural connection with the AcbSh or secondarily through changes in autonomic activity, stress, or circulating levels of orexigenic or satiety factors. In the present study, we used the immunohistochemical localization of Fos protein to map neuronal activation after unilateral muscimol injections into the AcbSh to determine whether AcbSh-mediated Fos expression remains lateralized in the circuit and whether secondary systemic changes in the rat can be excluded as primary factors in the activation of downstream component nuclei. Rats receiving only saline injections exhibited very little Fos immunoreactivity. In contrast, unilateral injections of muscimol into the AcbSh consistently increased Fos expression in several brain regions. Three distinct patterns of expression were observed. Fos synthesis in the LH was increased only on the side of the brain ipsilateral to the muscimol injection. Fos expression remained primarily ipsilateral to the injection site in the septohypothalamic, paraventricular hypothalamic (PVN), paratenial thalamic, and lateral habenular nuclei, and medial substantia nigra, but was increased bilaterally in the piriform cortex, supraoptic nucleus, central nucleus of the amygdala, and nucleus of the solitary tract. Smaller numbers of Fos-immunoreactive cells were seen unilaterally in the bed nucleus of the stria terminalis, medial ventral pallidum, arcuate nucleus, and ventral tegmental area and bilaterally in the supraoptic and tuberomammillary nuclei. The labeling in the LH, PVN, and other unilaterally labeled structures provides evidence that these brain regions are components of an AcbSh-mediated neural circuit and suggests that they may be involved in the expression of AcbSh-mediated feeding behavior. |
doi_str_mv | 10.1016/j.brainres.2005.05.002 |
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We have demonstrated previously that this treatment activates a number of brain regions, most significantly the lateral hypothalamus (LH). This activation could be elicited through a direct neural connection with the AcbSh or secondarily through changes in autonomic activity, stress, or circulating levels of orexigenic or satiety factors. In the present study, we used the immunohistochemical localization of Fos protein to map neuronal activation after unilateral muscimol injections into the AcbSh to determine whether AcbSh-mediated Fos expression remains lateralized in the circuit and whether secondary systemic changes in the rat can be excluded as primary factors in the activation of downstream component nuclei. Rats receiving only saline injections exhibited very little Fos immunoreactivity. In contrast, unilateral injections of muscimol into the AcbSh consistently increased Fos expression in several brain regions. Three distinct patterns of expression were observed. Fos synthesis in the LH was increased only on the side of the brain ipsilateral to the muscimol injection. Fos expression remained primarily ipsilateral to the injection site in the septohypothalamic, paraventricular hypothalamic (PVN), paratenial thalamic, and lateral habenular nuclei, and medial substantia nigra, but was increased bilaterally in the piriform cortex, supraoptic nucleus, central nucleus of the amygdala, and nucleus of the solitary tract. Smaller numbers of Fos-immunoreactive cells were seen unilaterally in the bed nucleus of the stria terminalis, medial ventral pallidum, arcuate nucleus, and ventral tegmental area and bilaterally in the supraoptic and tuberomammillary nuclei. 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Psychology ; GABA ; GABA Agonists - pharmacology ; Immunohistochemistry - methods ; Lateral hypothalamic area ; Male ; Microinjections - methods ; Muscimol - pharmacology ; Nerve Net - drug effects ; Nerve Net - physiology ; Nucleus Accumbens - drug effects ; Nucleus Accumbens - physiology ; Oncogene Proteins v-fos - metabolism ; Random Allocation ; Rat ; Rats ; Rats, Sprague-Dawley ; Time Factors ; Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><ispartof>Brain research, 2005-06, Vol.1048 (1), p.241-250</ispartof><rights>2005 Elsevier B.V.</rights><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c458t-7b044be14a2c996d40d237a0e6a8205b9c7a7e2926781a08e02dee589b0642d3</citedby><cites>FETCH-LOGICAL-c458t-7b044be14a2c996d40d237a0e6a8205b9c7a7e2926781a08e02dee589b0642d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16884599$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15921658$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Stratford, Thomas R.</creatorcontrib><title>Activation of feeding-related neural circuitry after unilateral injections of muscimol into the nucleus accumbens shell</title><title>Brain research</title><addtitle>Brain Res</addtitle><description>Chemical inhibition of neurons in the nucleus accumbens shell (AcbSh) elicits intense, behaviorally specific, feeding in satiated rats. We have demonstrated previously that this treatment activates a number of brain regions, most significantly the lateral hypothalamus (LH). This activation could be elicited through a direct neural connection with the AcbSh or secondarily through changes in autonomic activity, stress, or circulating levels of orexigenic or satiety factors. In the present study, we used the immunohistochemical localization of Fos protein to map neuronal activation after unilateral muscimol injections into the AcbSh to determine whether AcbSh-mediated Fos expression remains lateralized in the circuit and whether secondary systemic changes in the rat can be excluded as primary factors in the activation of downstream component nuclei. Rats receiving only saline injections exhibited very little Fos immunoreactivity. In contrast, unilateral injections of muscimol into the AcbSh consistently increased Fos expression in several brain regions. Three distinct patterns of expression were observed. Fos synthesis in the LH was increased only on the side of the brain ipsilateral to the muscimol injection. Fos expression remained primarily ipsilateral to the injection site in the septohypothalamic, paraventricular hypothalamic (PVN), paratenial thalamic, and lateral habenular nuclei, and medial substantia nigra, but was increased bilaterally in the piriform cortex, supraoptic nucleus, central nucleus of the amygdala, and nucleus of the solitary tract. Smaller numbers of Fos-immunoreactive cells were seen unilaterally in the bed nucleus of the stria terminalis, medial ventral pallidum, arcuate nucleus, and ventral tegmental area and bilaterally in the supraoptic and tuberomammillary nuclei. The labeling in the LH, PVN, and other unilaterally labeled structures provides evidence that these brain regions are components of an AcbSh-mediated neural circuit and suggests that they may be involved in the expression of AcbSh-mediated feeding behavior.</description><subject>Analysis of Variance</subject><subject>Animals</subject><subject>Behavior, Animal</subject><subject>Biological and medical sciences</subject><subject>Cell Count - methods</subject><subject>Drinking - drug effects</subject><subject>Eating - drug effects</subject><subject>Feeding behavior</subject><subject>Feeding Behavior - drug effects</subject><subject>Feeding Behavior - physiology</subject><subject>Feeding. Feeding behavior</subject><subject>Food intake</subject><subject>Fos</subject><subject>Functional Laterality - physiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GABA</subject><subject>GABA Agonists - pharmacology</subject><subject>Immunohistochemistry - methods</subject><subject>Lateral hypothalamic area</subject><subject>Male</subject><subject>Microinjections - methods</subject><subject>Muscimol - pharmacology</subject><subject>Nerve Net - drug effects</subject><subject>Nerve Net - physiology</subject><subject>Nucleus Accumbens - drug effects</subject><subject>Nucleus Accumbens - physiology</subject><subject>Oncogene Proteins v-fos - metabolism</subject><subject>Random Allocation</subject><subject>Rat</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Time Factors</subject><subject>Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><issn>0006-8993</issn><issn>1872-6240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNqFkUtr3DAQgEVpabbb_oXgS3vzdiTLetwaQvqAQC-5C1keN1psOZWslPz7St0tOQYGhDTfzIj5CLmkcKBAxefjYYjWh4jpwAD6Qw1gr8iOKslawTi8JjsAEK3Sursg71I6lmvXaXhLLmivGRW92pE_V27zj3bza2jWqZkQRx9-tRFnu-HYBMzRzo3z0WW_xafGThvGJgdf8zXlwxFdLU-1fsnJ-WWtz9vabPfYhOxmzKmxzuVlwIKle5zn9-TNZOeEH87nntx9vbm7_t7e_vz24_rqtnW8V1srB-B8QMotc1qLkcPIOmkBhVUM-kE7aSUyzYRU1IJCYCNir_QAgrOx25NPp7YPcf2dMW1m8cmV-TbgmpMRUnNZ9_ISyEBRTrV8EaSyqx1ZAcUJdHFNKeJkHqJfbHwyFEx1aI7mv0NTHZoa_wovzxPysOD4XHaWVoCPZ8AmZ-cp2uB8euaEUrwv1vfky4nDsuBHj9EUORhcURyLMzOu_qW__AUDBb9y</recordid><startdate>20050628</startdate><enddate>20050628</enddate><creator>Stratford, Thomas R.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>20050628</creationdate><title>Activation of feeding-related neural circuitry after unilateral injections of muscimol into the nucleus accumbens shell</title><author>Stratford, Thomas R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c458t-7b044be14a2c996d40d237a0e6a8205b9c7a7e2926781a08e02dee589b0642d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Analysis of Variance</topic><topic>Animals</topic><topic>Behavior, Animal</topic><topic>Biological and medical sciences</topic><topic>Cell Count - methods</topic><topic>Drinking - drug effects</topic><topic>Eating - drug effects</topic><topic>Feeding behavior</topic><topic>Feeding Behavior - drug effects</topic><topic>Feeding Behavior - physiology</topic><topic>Feeding. Feeding behavior</topic><topic>Food intake</topic><topic>Fos</topic><topic>Functional Laterality - physiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GABA</topic><topic>GABA Agonists - pharmacology</topic><topic>Immunohistochemistry - methods</topic><topic>Lateral hypothalamic area</topic><topic>Male</topic><topic>Microinjections - methods</topic><topic>Muscimol - pharmacology</topic><topic>Nerve Net - drug effects</topic><topic>Nerve Net - physiology</topic><topic>Nucleus Accumbens - drug effects</topic><topic>Nucleus Accumbens - physiology</topic><topic>Oncogene Proteins v-fos - metabolism</topic><topic>Random Allocation</topic><topic>Rat</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Time Factors</topic><topic>Vertebrates: anatomy and physiology, studies on body, several organs or systems</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Stratford, Thomas R.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Brain research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Stratford, Thomas R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Activation of feeding-related neural circuitry after unilateral injections of muscimol into the nucleus accumbens shell</atitle><jtitle>Brain research</jtitle><addtitle>Brain Res</addtitle><date>2005-06-28</date><risdate>2005</risdate><volume>1048</volume><issue>1</issue><spage>241</spage><epage>250</epage><pages>241-250</pages><issn>0006-8993</issn><eissn>1872-6240</eissn><coden>BRREAP</coden><abstract>Chemical inhibition of neurons in the nucleus accumbens shell (AcbSh) elicits intense, behaviorally specific, feeding in satiated rats. We have demonstrated previously that this treatment activates a number of brain regions, most significantly the lateral hypothalamus (LH). This activation could be elicited through a direct neural connection with the AcbSh or secondarily through changes in autonomic activity, stress, or circulating levels of orexigenic or satiety factors. In the present study, we used the immunohistochemical localization of Fos protein to map neuronal activation after unilateral muscimol injections into the AcbSh to determine whether AcbSh-mediated Fos expression remains lateralized in the circuit and whether secondary systemic changes in the rat can be excluded as primary factors in the activation of downstream component nuclei. Rats receiving only saline injections exhibited very little Fos immunoreactivity. In contrast, unilateral injections of muscimol into the AcbSh consistently increased Fos expression in several brain regions. Three distinct patterns of expression were observed. Fos synthesis in the LH was increased only on the side of the brain ipsilateral to the muscimol injection. Fos expression remained primarily ipsilateral to the injection site in the septohypothalamic, paraventricular hypothalamic (PVN), paratenial thalamic, and lateral habenular nuclei, and medial substantia nigra, but was increased bilaterally in the piriform cortex, supraoptic nucleus, central nucleus of the amygdala, and nucleus of the solitary tract. Smaller numbers of Fos-immunoreactive cells were seen unilaterally in the bed nucleus of the stria terminalis, medial ventral pallidum, arcuate nucleus, and ventral tegmental area and bilaterally in the supraoptic and tuberomammillary nuclei. The labeling in the LH, PVN, and other unilaterally labeled structures provides evidence that these brain regions are components of an AcbSh-mediated neural circuit and suggests that they may be involved in the expression of AcbSh-mediated feeding behavior.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>15921658</pmid><doi>10.1016/j.brainres.2005.05.002</doi><tpages>10</tpages></addata></record> |
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subjects | Analysis of Variance Animals Behavior, Animal Biological and medical sciences Cell Count - methods Drinking - drug effects Eating - drug effects Feeding behavior Feeding Behavior - drug effects Feeding Behavior - physiology Feeding. Feeding behavior Food intake Fos Functional Laterality - physiology Fundamental and applied biological sciences. Psychology GABA GABA Agonists - pharmacology Immunohistochemistry - methods Lateral hypothalamic area Male Microinjections - methods Muscimol - pharmacology Nerve Net - drug effects Nerve Net - physiology Nucleus Accumbens - drug effects Nucleus Accumbens - physiology Oncogene Proteins v-fos - metabolism Random Allocation Rat Rats Rats, Sprague-Dawley Time Factors Vertebrates: anatomy and physiology, studies on body, several organs or systems |
title | Activation of feeding-related neural circuitry after unilateral injections of muscimol into the nucleus accumbens shell |
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