Digital Readout of Target Binding with Attomole Detection Limits via Enzyme Amplification in Femtoliter Arrays

In this communication, single molecules of β-galactosidase were captured on a 1 mm femtoliter array using biotin−streptavidin binding. The femtoliter arrays, containing 24 000 individual reaction chambers, permit digital concentration readout as the percentage of reaction vessels that successfully c...

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Bibliographic Details
Published in:Journal of the American Chemical Society 2006-05, Vol.128 (19), p.6286-6287
Main Authors: Rissin, David M, Walt, David R
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
beta-Galactosidase - analysis
Biological and medical sciences
Biotin
Fundamental and applied biological sciences. Psychology
General aspects, investigation methods
Immunoenzyme Techniques
Microarray Analysis
Nanotechnology - instrumentation
Nanotechnology - methods
Nanotechnology - standards
Nucleic acids
Sensitivity and Specificity
Streptavidin
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Summary:In this communication, single molecules of β-galactosidase were captured on a 1 mm femtoliter array using biotin−streptavidin binding. The femtoliter arrays, containing 24 000 individual reaction chambers, permit digital concentration readout as the percentage of reaction vessels that successfully capture a target molecule is correlated to the bulk target concentration. This capture and readout approach should prove useful for DNA and antibody assays that utilize an enzyme label to catalyze the generation of a fluorescent signal.
ISSN:0002-7863
1520-5126
DOI:10.1021/ja058425e