Antileukemic Effect of Interleukin-7-Transduced Bone Marrow Stromal Cells in Mice Following Allogeneic T-Cell-Depleted Bone Marrow Transplantation

Impaired immune reconstitution following allogeneic bone marrow transplantation (BMT) remains a major obstacle to its clinical application. In this study, interleukin (IL)-7–transduced bone marrow stromal cells (MSC-IL7, 1 × 10 6/mouse) were transfused into lethally irradiated C57BL/6 recipient mice...

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Bibliographic Details
Published in:Transplantation proceedings 2005-06, Vol.37 (5), p.2297-2299
Main Authors: Li, A.L., Li, C., Feng, Y.G., Yuan, G.H., Wang, G.M., Hao, J., Gao, X., Xie, S.S.
Format: Article
Language:English
Subjects:
Animals
Bone Marrow Cells - cytology
Bone Marrow Transplantation - immunology
Bone Marrow Transplantation - mortality
Genetic Therapy - methods
Humans
Hypersensitivity, Delayed
Interleukin-7 - genetics
Interleukin-7 - immunology
Leukemia, Experimental - immunology
Leukemia, Experimental - mortality
Leukemia, Experimental - prevention & control
Leukemia, Experimental - therapy
Lymphocyte Depletion
Mice
Mice, Inbred C57BL
Reverse Transcriptase Polymerase Chain Reaction
Stromal Cells - immunology
Stromal Cells - transplantation
Survival Analysis
Transduction, Genetic
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Summary:Impaired immune reconstitution following allogeneic bone marrow transplantation (BMT) remains a major obstacle to its clinical application. In this study, interleukin (IL)-7–transduced bone marrow stromal cells (MSC-IL7, 1 × 10 6/mouse) were transfused into lethally irradiated C57BL/6 recipient mice. By day 40 after transplantation, the recipient mice were challenged with the lymphoma cell line EL4. MSC-IL7 cotransplantation protected recipient mice from leukemic mortality (MST >120 days after BMT vs mean survival time (MST) 70 days in the PBS group) It enhance the PFC count and DTH responses of recipients after transplantation. In conclusion, MSC mediated IL-7 gene therapy and may be a more feasible strategy to restore immune function following allo-TCD-BMT.
ISSN:0041-1345
1873-2623
DOI:10.1016/j.transproceed.2005.03.088