Pseudomonas aeruginosa infection in the hypoventilated middle ear: An experimental model

Conclusion This is a suitable model for studying different aspects of the pathophysiology of chronic suppurative otitis media. Objective To analyze the methodological features of an animal model of chronic suppurative otitis media induced by intratympanic inoculation of Pseudomonas aeruginosa. Mater...

Full description

Saved in:
Bibliographic Details
Published in:Acta oto-laryngologica 2005-03, Vol.125 (3), p.266-269
Main Authors: Trinidad, Almudena, Ramírez-Camacho, Rafael, García-Berrocal, Jose Ramón, María Verdaguer, José, Vicente, Javier, Pinilla, María Teresa
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Blister - microbiology
Blister - pathology
Bulla
Chronic otitis media
Disease Models, Animal
Ear, Inner - microbiology
Ear, Inner - pathology
Eustachian tube obstruction
Male
Medical sciences
Microscopy
Otitis Media with Effusion - microbiology
Otitis Media with Effusion - pathology
Otorhinolaryngology. Stomatology
Otoscopy
Pseudomonas aeruginosa
Pseudomonas Infections - complications
Rats
Rats, Wistar
Temporal Bone - microbiology
Temporal Bone - pathology
Tympanic Membrane - microbiology
Tympanic Membrane - pathology
Wistar rat
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Conclusion This is a suitable model for studying different aspects of the pathophysiology of chronic suppurative otitis media. Objective To analyze the methodological features of an animal model of chronic suppurative otitis media induced by intratympanic inoculation of Pseudomonas aeruginosa. Material and methods Otitis media was induced by inoculation of P. aeruginosa through the inferior aspect of the rat bulla and cauterization of the Eustachian tube via a transpalatal approach. Inspection of the tympanic membrane, culturing of middle ear effusion and processing of the temporal bones for light microscopy were performed. Results Abnormal otomicroscopic findings and persistence of infection were correlated with the histopathological changes found in middle ear tissues.
ISSN:0001-6489
1651-2251
DOI:10.1080/00016480410022804