Single-Molecule Experiments in Synthetic Biology: An Approach to the Affinity Ranking of DNA-Binding Peptides

Peptide–DNA recognition: The specific binding of peptides to DNA representing a section of the binding sequence of a transcription activator has been demonstrated for the first time at the single‐molecule level with AFM (experimental principle, see picture). The results underscore the importance of...

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Bibliographic Details
Published in:Angewandte Chemie International Edition 2005-06, Vol.44 (25), p.3921-3924
Main Authors: Eckel, Rainer, Wilking, Sven David, Becker, Anke, Sewald, Norbert, Ros, Robert, Anselmetti, Dario
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Bacterial Proteins - chemical synthesis
Bacterial Proteins - chemistry
Crystallography, X-Ray
DNA - chemistry
Models, Molecular
molecular recognition
Molecular Sequence Data
peptides
Peptides - chemical synthesis
Peptides - chemistry
point mutations
Protein Binding
Protein Conformation
Protein Structure, Tertiary
scanning probe microscopy
single-molecule studies
Surface Properties
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Description
Summary:Peptide–DNA recognition: The specific binding of peptides to DNA representing a section of the binding sequence of a transcription activator has been demonstrated for the first time at the single‐molecule level with AFM (experimental principle, see picture). The results underscore the importance of peptide length and cooperative effects as well as the contribution of single‐point mutations on specific binding.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.200500152