Physicochemical characterization of poly( l-lactic acid) and poly( d, l-lactide-co-glycolide) nanoparticles with polyethylenimine as gene delivery carrier

Polymer nanoparticles have been used as non-viral gene delivery systems and drug delivery systems. In this study, biodegradable poly( l-lactic acid) (PLA)/polyethylenimine (PEI) and poly( d, l-lactide-co-glycolide) (PLGA)/PEI nanoparticles were prepared and characterized as gene delivery systems. Th...

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Bibliographic Details
Published in:International journal of pharmaceutics 2005-07, Vol.298 (1), p.255-262
Main Authors: Kim, In-Sook, Lee, Soo-Kyung, Park, Yu-Mi, Lee, Yong-Bok, Shin, Sang-Chul, Lee, Kang Choon, Oh, In-Joon
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cell Survival
Drug Delivery Systems
Drug Stability
Gene delivery
Gene Transfer Techniques
General pharmacology
Genetic Vectors
Lactic Acid - administration & dosage
Lactic Acid - chemistry
Medical sciences
Nanoparticles
Nanostructures
Particle Size
Pharmaceutical technology. Pharmaceutical industry
Pharmacology. Drug treatments
Poly( d, l-lactide-co-glycolide)
Poly( l-lactic acid)
Polyesters
Polyethyleneimine - administration & dosage
Polyethyleneimine - chemistry
Polyethylenimine
Polyglycolic Acid
Polymers - administration & dosage
Polymers - chemistry
Transfection
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Summary:Polymer nanoparticles have been used as non-viral gene delivery systems and drug delivery systems. In this study, biodegradable poly( l-lactic acid) (PLA)/polyethylenimine (PEI) and poly( d, l-lactide-co-glycolide) (PLGA)/PEI nanoparticles were prepared and characterized as gene delivery systems. The PLA/PEI and PLGA/PEI nanoparticles, which were prepared by a diafiltration method, had spherical shapes and smooth surface characteristics. The size of nanoparticles was controlled by the amount of PEI, which acted as a hydrophilic moiety, which effectively reduced the interfacial energy between the particle surface and the aqueous media. The nanoparticles showed an excellent dispersive stability under storage in a phosphate-buffered saline solution for 12 days. The positive zeta-potentials for the nanoparticles decreased and changed to negative values with increasing plasmid DNA (pDNA) content. Agarose gel electrophoresis showed that the complex formation between the nanoparticles and the pDNA coincided with the zeta-potential results. The results of in vitro transfection and cell viability on HEK 293 cells indicated that the nanoparticles could be used as gene delivery carriers.
ISSN:0378-5173
1873-3476
DOI:10.1016/j.ijpharm.2005.04.017