Hypoxia-inducible factor-1alpha and the glycolytic phenotype in tumors

Metastatic tumors generally exhibit aerobic glycolysis (the Warburg effect). The advent of [18F]fluorodeoxyglucose positron emission tomography imaging, coupled with recent findings linking hypoxia-inducible factor (HIF-1alpha) overexpression to aggressive cancers, has rekindled an interest in this...

Full description

Saved in:
Bibliographic Details
Published in:Neoplasia (New York, N.Y.) N.Y.), 2005-04, Vol.7 (4), p.324-330
Main Authors: Robey, Ian F, Lien, Anthony D, Welsh, Sarah J, Baggett, Brenda K, Gillies, Robert J
Format: Article
Language:English
Subjects:
Blotting, Western
Cell Line, Tumor
DNA Primers - chemistry
Gene Expression Regulation, Neoplastic
Glucose - metabolism
Glucose - pharmacokinetics
Glycolysis
Humans
Hypoxia
Hypoxia-Inducible Factor 1, alpha Subunit
Lactates - metabolism
Neoplasms - metabolism
Phenotype
Reverse Transcriptase Polymerase Chain Reaction
Time Factors
Transcription Factors - metabolism
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Metastatic tumors generally exhibit aerobic glycolysis (the Warburg effect). The advent of [18F]fluorodeoxyglucose positron emission tomography imaging, coupled with recent findings linking hypoxia-inducible factor (HIF-1alpha) overexpression to aggressive cancers, has rekindled an interest in this aspect of tumor metabolism. These studies explore the role of HIF-1alpha in human breast cancer lines and its relationship to glycolytic regulation. Here we demonstrate that, under normal oxygen conditions, nonmetastatic cells consume less glucose and express low HIF-1alpha, whereas metastatic cells constitutively express high glycolysis and HIF-1alpha, suggesting that dysregulation of HIF-1alpha may induce the Warburg effect. This hypothesis was tested by renormalizing HIF-1alpha levels in renal carcinoma cells, leading to inhibition of aerobic glycolysis.
ISSN:1522-8002