Virulence Attenuation and Live Vaccine Potential of aroA, crp cdt cya, and Plasmid-Cured Mutants of Salmonella enterica Serovar Abortusovis in Mice and Sheep

Three live vaccine candidates of Salmonella enterica subspecies I serotype Abortusovis (aroA, cya crp cdt, and plasmid-cured strains) have been developed, and their efficacies in inducing humoral antibodies and protecting against abortion after challenge with wild-type strain SS44 were evaluated in...

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Bibliographic Details
Published in:Infection and Immunity 2005-07, Vol.73 (7), p.4302-4308
Main Authors: Uzzau, Sergio, Marogna, Gavino, Leori, Guido Sisinnio, Curtiss, Roy, Schianchi, Giuseppe, Stocker, Bruce A. D, Rubino, Salvatore
Format: Article
Language:English
Subjects:
Abortion, Veterinary - prevention & control
Animals
Applied microbiology
Bacterial Infections
Bacteriology
Biological and medical sciences
Female
Fundamental and applied biological sciences. Psychology
Mice
Mice, Inbred BALB C
Microbiology
Miscellaneous
Mutation
Plasmids
Pregnancy
Salmonella enterica - immunology
Salmonella Infections, Animal - prevention & control
Salmonella Vaccines - immunology
Sheep
Typhoid-Paratyphoid Vaccines - immunology
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
Vaccines, Attenuated - immunology
Virulence
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Summary:Three live vaccine candidates of Salmonella enterica subspecies I serotype Abortusovis (aroA, cya crp cdt, and plasmid-cured strains) have been developed, and their efficacies in inducing humoral antibodies and protecting against abortion after challenge with wild-type strain SS44 were evaluated in sheep. Following estrus synchronization, animals were immunized 3 weeks after fertilization and boosted once 3 weeks later. Following challenge with wild-type SS44, pregnancy failure of vaccinated ewes was reduced compared to that of nonimmunized controls. Attenuation of each vaccine was also assessed in challenge experiments with nonimmunized pregnant ewes and in BALB/c mice. All three vaccine candidates appear to be safe for use in sheep and provide a model for the development of live vaccine candidates against naturally occurring ovine salmonellosis.
ISSN:0019-9567
1098-5522
DOI:10.1128/IAI.73.7.4302-4308.2005