Tubulin is the target binding site for NAP-related peptides: ADNF-9, D-NAP, and D-SAL

The authors set out to investigate whether NAP-related peptides interact with tubulin at a NAP binding site. Previous studies have shown that the neuroprotective peptide NAP binds to tubulin. As NAP (NAPVSIPQ) shares structural similarities with ADNF-9 (SALLRSIPA), and the all-D-enantiomers, D-NAP a...

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Bibliographic Details
Published in:Journal of molecular neuroscience 2006-01, Vol.28 (3), p.303-308
Main Authors: Holtser-Cochav, Miri, Divinski, Inna, Gozes, Illana
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Animals, Newborn
Binding Sites
Brain Chemistry
Chromatography, Affinity
Competition
Molecular Sequence Data
Neuroprotective Agents - metabolism
Oligopeptides - genetics
Oligopeptides - metabolism
Peptides
Peptides - genetics
Peptides - metabolism
Rats
Tubulin - chemistry
Tubulin - metabolism
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Summary:The authors set out to investigate whether NAP-related peptides interact with tubulin at a NAP binding site. Previous studies have shown that the neuroprotective peptide NAP binds to tubulin. As NAP (NAPVSIPQ) shares structural similarities with ADNF-9 (SALLRSIPA), and the all-D-enantiomers, D-NAP and D-SAL, it was hypothesized that all of these peptides compete with NAP-tubulin binding. Using NAP affinity column and extracts from newborn rat brain (cerebral cortex), we now show that the above-mentioned peptides compete with NAP binding to tubulin. The identification of tubulin as a target binding site for NAP-related peptides explains, in part, the broad neuroprotective activity offered by these potent peptides.
ISSN:0895-8696
0895-8696
1559-1166
DOI:10.1385/JMN:28:3:303